Document Detail


Generation of Epitope-Tagged GPCRs.
MedLine Citation:
PMID:  21607852     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The addition of one or more epitope tags to G-protein-coupled receptors (GPCRs) has facilitated a wide variety of studies on their structure and function. Epitope-tagging is achieved using relatively straightforward molecular techniques but requires careful consideration about the nature of the epitope tag and its location within the receptor. Here, we describe both the strategies and methodologies for the generation of epitope-tagged GPCRs. We highlight a range of possible techniques that depend upon the available starting material, the nature of the epitope to be incorporated, and suggest a strategy to ease the tagging of multiple receptor types.
Authors:
Yan Huang; Gary B Willars
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  746     ISSN:  1940-6029     ISO Abbreviation:  -     Publication Date:  2011  
Date Detail:
Created Date:  2011-5-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  53-84     Citation Subset:  -    
Affiliation:
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