Document Detail


Generation of a Cre Knock-in into the Myocardin locus to mark early cardiac and smooth muscle cell lineages.
MedLine Citation:
PMID:  25174608     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The molecular events that control cell fate determination in cardiac and smooth muscle lineages remain elusive. Myocardin is an important transcription co-factor that regulates cell proliferation, differentiation and development of the cardiovascular system. Here, we describe the construction and analysis of a dual Cre and Enhanced Green Fluorescent Protein (EGFP) knock-in mouse line in the Myocardin locus (Myocd(KI) ). We report that the Myocd(KI) allele expresses the Cre enzyme and the EGFP in a manner that recapitulates endogenous Myocardin expression patterns. We show that Myocardin expression marks the earliest cardiac and smooth muscle lineages. Furthermore, this genetic model allows for the identification of a cardiac cell population which maintains both Myocardin and Isl-1 expression, in E7.75 - E8.0 embryos, highlighting the contributions and merge of the first and second heart fields during cardiogenesis. Therefore, the Myocd(KI) allele is a unique tool for studying cardiovascular development and lineage-specific gene manipulation. © 2014 Wiley Periodicals, Inc.
Authors:
Ramón A Espinoza-Lewis; Da-Zhi Wang
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-8-30
Journal Detail:
Title:  Genesis (New York, N.Y. : 2000)     Volume:  -     ISSN:  1526-968X     ISO Abbreviation:  Genesis     Publication Date:  2014 Aug 
Date Detail:
Created Date:  2014-9-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100931242     Medline TA:  Genesis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Wiley Periodicals, Inc., a Wiley company.
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