Document Detail


Generation and characterization of human cryptorchid-specific induced pluripotent stem cells from urine.
MedLine Citation:
PMID:  23025704     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cryptorchidism is a common congenital birth defect in human beings with the possible complication of infertility. An in vitro model of cryptorchidism might be valuable due to the inaccessibility of human embryos for research purposes. In this study, we reprogrammed urine cells containing genetic variations in insulin-like factor 3, zinc finger (ZNF) 214, and ZNF215 from a cryptorchid patient by introducing human OCT4, SOX2, C-MYC, and KLF4 with lentivirus. The cells were then replated on irradiated mouse embryonic fibroblasts and cultured with the human embryonic stem (ES) cell medium. The compact colonies with well-defined borders were manually picked, and 2 induced pluripotent cell lines were fully characterized. Our results demonstrated that these 2 cell lines were similar to human ES cells in morphological appearance, marker expression, and epigenetic status of the pluripotent cell-specific gene, OCT4. These cells could be differentiated into cells of all 3 germ layers in teratomas and in vitro, including into the VASA-positive germ cell lineage. Both parental urine cells and the reprogrammed cells possessed the normal karyotype and the same short tandem repeat loci, indicating that these 2 cell population share the same genetic identity. This establishment and characterization of human urine-derived cryptorchid-specific induced pluripotent stem cells could present a good human genetic system for future studies investigating the molecular mechanism of cryptorchidism.
Authors:
Junmei Zhou; Xue Wang; Shengli Zhang; Yijun Gu; Ling Yu; Jing Wu; Tongbin Gao; Fang Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-01-04
Journal Detail:
Title:  Stem cells and development     Volume:  22     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-15     Completed Date:  2013-08-05     Revised Date:  2014-03-09    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  717-25     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques
Cell Differentiation*
Cell Lineage
Cryptorchidism / metabolism,  pathology*
DNA-Binding Proteins / genetics
Genetic Variation
Genetic Vectors
Germ Layers
Humans
Induced Pluripotent Stem Cells / cytology*,  metabolism
Insulin / genetics*
Kruppel-Like Transcription Factors / genetics
Lentivirus / genetics
Male
Mutation
Octamer Transcription Factor-3 / genetics
Proteins / genetics*
Proto-Oncogene Proteins c-myc / genetics
SOXB1 Transcription Factors / genetics
Urine / cytology*
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/GKLF protein; 0/Insulin; 0/Kruppel-Like Transcription Factors; 0/Leydig insulin-like protein; 0/MYC protein, human; 0/Octamer Transcription Factor-3; 0/POU5F1 protein, human; 0/Proteins; 0/Proto-Oncogene Proteins c-myc; 0/SOX2 protein, human; 0/SOXB1 Transcription Factors; 0/ZNF214 protein, human; 0/ZNF215 protein, human
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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