| Generation of bioengineered corneas with decellularized xenografts and human keratocytes. | |
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MedLine Citation:
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PMID: 20739475 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: Decellularization of animal corneas is a promising method for the development of artificial human corneas by tissue engineering. In this study, two different decellularization protocols were evaluated to determine which one is able to best preserve the histologic structure, composition, and optical behavior of decellularized porcine corneas. Then, these corneas were recellularized with human keratocytes to obtain a partial human corneal substitute. METHODS: Two different decellularization protocols were applied, using NaCl and SDS, to determine which one is able to best preserve the histologic structure, composition, and optical behavior of the decellularized corneas. Then, those decellularized corneas that showed the most appropriate results were recellularized with human keratocytes and evaluated at the histologic, biochemical, and optical levels for use in regenerative medicine. RESULTS: The results showed that 1.5 M NaCl treatment of porcine corneas is able to generate an acellular corneal stroma with adequate histologic and optical properties and that human keratocytes are able to penetrate and spread within this scaffold with proper levels of cell differentiation. In contrast, 0.1% SDS treatment of porcine corneas resulted in high levels of fibril disorganization and poor optical behavior of these corneas. CONCLUSIONS: In conclusion, the authors suggest that the decellularization of animal corneas with 1.5 M NaCl represents a useful method for the development of human bioengineered corneas with therapeutic potential. |
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Authors:
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Miguel Gonzalez-Andrades; Juan de la Cruz Cardona; Ana Maria Ionescu; Antonio Campos; Maria Del Mar Perez; Miguel Alaminos |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-01-05 |
Journal Detail:
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Title: Investigative ophthalmology & visual science Volume: 52 ISSN: 1552-5783 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-06 Completed Date: 2011-02-07 Revised Date: 2011-03-04 |
Medline Journal Info:
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Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 215-22 Citation Subset: IM |
Affiliation:
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Department of Histology, University of Granada, Granada, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bioengineering / methods* Cell Differentiation Cell Separation Cell Transplantation* Cells, Cultured Cornea / drug effects* Corneal Stroma / cytology*, physiology Fibroblasts / transplantation* Humans Light Regenerative Medicine Scattering, Radiation Sodium Chloride / pharmacology* Sodium Dodecyl Sulfate / pharmacology* Swine Transplantation, Heterologous |
| Chemical | |
Reg. No./Substance:
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151-21-3/Sodium Dodecyl Sulfate; 7647-14-5/Sodium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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