Document Detail

Generalized blocking in S phase by methotrexate.
MedLine Citation:
PMID:  3419447     Owner:  NLM     Status:  MEDLINE    
An attempt was made to enhance the frequency of prometaphase cells for high-resolution-banding studies in untransformed Syrian hamster fibroblasts using a typical methotrexate (MTX) block/bromodeoxyuridine release schedule. The recovery 'wave' was serially sampled and detailed sub-phase analysis made using the replication bands resulting from bromodeoxyuridine uptake. Of the 3 batches of MTX used, one (Sigma greater than 2 years old) was found to have decayed to a non-toxic compound which produced almost no measurable perturbation of the cell cycle at any concentration used. The other 2 (new Sigma and new Lederle), whilst producing mitotic index fluctuations which could be construed to indicate blocking and "synchrony", gave absolutely no evidence of any specific blocking site, but rather a general stoppage (or slowing down) during MTX treatment and continuation in exactly the same order as untreated controls upon release.
J R Savage; R Prasad
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  201     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1988-10-21     Completed Date:  1988-10-21     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  195-201     Citation Subset:  IM    
Medical Research Council, Radiobiology Unit, Didcot, Oxon, Great Britain.
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MeSH Terms
Bromodeoxyuridine / pharmacology
Cell Survival / drug effects
Cells, Cultured
Fibroblasts / cytology,  drug effects
Interphase / drug effects*
Methotrexate / pharmacology*,  toxicity
Mitotic Index
Reg. No./Substance:
59-05-2/Methotrexate; 59-14-3/Bromodeoxyuridine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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