Document Detail

General Transduction in Vibrio cholerae.
MedLine Citation:
PMID:  7216473     Owner:  NLM     Status:  MEDLINE    
Evidence was obtained for general transduction in Vibrio cholerae. Transduction of three amino acid markers and three antibiotic resistance characters was demonstrated using strains of biotype eltor and biotype cholerae. Some of the genetic characters were transduced from a biotype eltor donor (and its mutant derivatives) to biotype cholerae and eltor recipients. For the genetic traits examined, the frequencies of transduction ranged between 10(-5) and 10(-8). Maximal frequencies were obtained with transducing phage lysates that were irradiated with ultraviolet light. The development of a system of general transduction will now aid in fine structure analysis and detailed mapping of the chromosome of V. cholerae.
J E Ogg; T L Timme; M M Alemohammad
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Infection and immunity     Volume:  31     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1981 Feb 
Date Detail:
Created Date:  1981-06-13     Completed Date:  1981-06-13     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  737-41     Citation Subset:  IM    
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MeSH Terms
Bacteriophages / genetics
Drug Resistance, Microbial
Transduction, Genetic*
Vibrio cholerae / genetics*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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