Document Detail


Modern pathology methods for neural investigations.
MedLine Citation:
PMID:  21212254     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This session at the 2010 joint symposium of the Society of Toxicologic Pathology (STP) and the International Federation of Societies of Toxicologic Pathologists (IFSTP) explored modern neuropathology methods for assessing the neurotoxicologic potential of xenobiotics. Conventional techniques to optimally prepare and evaluate the central and peripheral neural tissues while minimizing artifact were reviewed, and optimal schemes were set forth for evaluation of the nervous system during both routine (i.e., general toxicity) studies and enhanced (i.e., specialized neurotoxicity) studies. Stereology was introduced as the most appropriate means of examining the possible impact of toxicants on neural cell numbers. A focused discussion on brain sampling took place among a panel of expert neuroscientists (anatomists and pathologists) and the audience regarding the proper balance between sufficient sampling and cost- and time-effectiveness of the analysis. No consensus was reached on section orientation (coronal sections of both sides vs. a parasagittal longitudinal section with several unilateral hemisections from the contralateral side), but most panelists favored sampling at least 8 sections (or approximately double to triple the current complement) in routine toxicity studies.
Authors:
Sarah L Hale; Lydia Andrews-Jones; William H Jordan; Bernard S Jortner; Rogely W Boyce; John T Boyce; Robert C Switzer Iii; Mark T Butt; Robert H Garman; Karl Jensen; Georg Krinke; Peter B Little
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Publication Detail:
Type:  Journal Article     Date:  2011-01-06
Journal Detail:
Title:  Toxicologic pathology     Volume:  39     ISSN:  1533-1601     ISO Abbreviation:  Toxicol Pathol     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-03-07     Completed Date:  2011-06-23     Revised Date:  2011-07-11    
Medline Journal Info:
Nlm Unique ID:  7905907     Medline TA:  Toxicol Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  52-7     Citation Subset:  IM    
Affiliation:
Covance Laboratories, Inc., Vienna, VA, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Congresses as Topic
Evaluation Studies as Topic
Humans
Nervous System / anatomy & histology*
Nervous System Diseases / chemically induced*,  pathology*
Neurotoxicity Syndromes / pathology
Societies, Scientific
Toxicity Tests / methods*
Xenobiotics / toxicity*
Chemical
Reg. No./Substance:
0/Xenobiotics

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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