Document Detail


Gene transfer systems derived from Visna virus: analysis of virus production and infectivity.
MedLine Citation:
PMID:  11145895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Efficient transfer of therapeutic genes into nondividing human cells can be accomplished by inserting the genes into lentiviruses and infecting the cells with the modified viruses. The most developed lentivirus gene transfer systems are based on HIV-1, but because of the widespread HIV epidemic, the use of HIV-based vectors for gene therapy may be associated with a safety risk. In an attempt to find another lentivirus which can transduce human cells and might be safer than HIV-1, we generated gene transfer constructs based on the sheep lentivirus Visna. Molecular analysis of the constructs in a transient production system indicated that Visna produced as many mature virus particles as did HIV-1. Moreover, the virus particles incorporated a heterologous surface protein marker-gene-containing vector RNAs as efficiently as did HIV-1. However, the Visna virus transduced target cells poorly because of defects in reverse transcription and integration of the vector. Further modifications must be made to the Visna gene transfer system if the system is to be used in clinical gene therapy applications.
Authors:
R D Berkowitz; H Ilves; I Plavec; G Veres
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Virology     Volume:  279     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-01-26     Completed Date:  2001-03-01     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  116-29     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Affiliation:
SyStemix Incorporated, 3155 Porter Drive, Palo Alto, California, 94304, USA. docbob@slip.net
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Gene Therapy / methods
Gene Transfer Techniques*
Genetic Vectors* / genetics
Humans
Plasmids / genetics
RNA, Viral / metabolism
Transcription, Genetic
Transfection
Virus Assembly
Virus Integration
Visna / virology
Visna-maedi virus / genetics*,  pathogenicity,  physiology
Chemical
Reg. No./Substance:
0/RNA, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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