| Gene transfer for ischemic heart failure in a preclinical model. | |
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MedLine Citation:
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PMID: 21633324 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Various emerging technologies are being developed for patients with heart failure. Well-established preclinical evaluations are necessary to determine their efficacy and safety. Gene therapy using viral vectors is one of the most promising approaches for treating cardiac diseases. Viral delivery of various different genes by changing the carrier gene has immeasurable therapeutic potential. In this video, the full process of an animal model of heart failure creation followed by gene transfer is presented using a swine model. First, myocardial infarction is created by occluding the proximal left anterior descending coronary artery. Heart remodeling results in chronic heart failure. Unique to our model is a fairly large scar which truly reflects patients with severe heart failure who require aggressive therapy for positive outcomes. After myocardial infarct creation and development of scar tissue, an intracoronary injection of virus is demonstrated with simultaneous nitroglycerine infusion. Our injection method provides simple and efficient gene transfer with enhanced gene expression. This combination of a myocardial infarct swine model with intracoronary virus delivery has proven to be a consistent and reproducible methodology, which helps not only to test the effect of individual gene, but also compare the efficacy of many genes as therapeutic candidates. |
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Authors:
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Kiyotake Ishikawa; Dennis Ladage; Lisa Tilemann; Kenneth Fish; Yoshiaki Kawase; Roger J Hajjar |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media Date: 2011-05-15 |
Journal Detail:
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Title: Journal of visualized experiments : JoVE Volume: - ISSN: 1940-087X ISO Abbreviation: J Vis Exp Publication Date: 2011 |
Date Detail:
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Created Date: 2011-06-02 Completed Date: 2012-04-06 Revised Date: 2013-05-24 |
Medline Journal Info:
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Nlm Unique ID: 101313252 Medline TA: J Vis Exp Country: United States |
Other Details:
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Languages: eng Pagination: - Citation Subset: IM |
Affiliation:
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Cardiovascular Research Center, Mount Sinai School of Medicine. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics Animals Disease Models, Animal* Female Gene Transfer Techniques* Heart Failure / genetics, therapy Myocardial Infarction / genetics, therapy Myocardial Ischemia / genetics*, therapy Swine |
| Grant Support | |
ID/Acronym/Agency:
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HL071763/HL/NHLBI NIH HHS; HL080498/HL/NHLBI NIH HHS; HL083156/HL/NHLBI NIH HHS; HL088434/HL/NHLBI NIH HHS; P20HL100396/HL/NHLBI NIH HHS; R01 HL093183/HL/NHLBI NIH HHS |
| Comments/Corrections | |
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