| Gene transfer into coronary arteries of intact animals with a percutaneous balloon catheter. | |
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MedLine Citation:
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PMID: 1606665 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Genetic manipulation of the vasculature may offer insights into the pathogenesis of coronary artery disease and may lead to gene therapy for disorders such as restenosis after percutaneous coronary angioplasty. The goal of this study was to develop a percutaneous method for gene transfer into coronary arteries of intact animals. Liposomes were used to facilitate transfection in coronary arteries with a plasmid containing the cDNA encoding luciferase. This reporter was chosen since it is not expressed in mammalian cells, and it can be quantified using a sensitive assay (light production). Mongrel dogs were catheterized, and DNA was delivered to coronary arteries via a porous perfusion balloon system. Luciferase expression was measured 3-5 days after the procedure, when the dogs were killed. Luciferase activity in control arteries (n = 12) was no higher than average background activity. Eight of 12 transfected arteries exhibited gene expression, averaging 4.3 +/- 2.1 pg luciferase (p less than 0.01, transfected versus control arteries). In addition, the ability to transfect DNA into femoral arteries without a transfection vehicle was tested. Five dogs were subjected to surgical transfection attempts in their femoral arteries with either DNA alone or DNA plus liposomes. Luciferase was expressed in all 10 femoral arteries; those treated with DNA alone expressed 35.6 +/- 8 pg luciferase, and those treated with DNA plus liposomes expressed 42.3 +/- 14 pg luciferase (p = 0.70). These results demonstrate the use of a percutaneous catheter to achieve gene transfer and expression in coronary arteries of intact dogs and suggest that the efficiency of intra-arterial gene transfer may be similar whether or not a transfection vehicle is used. |
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Authors:
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G D Chapman; C S Lim; R S Gammon; S C Culp; J S Desper; R P Bauman; J L Swain; R S Stack |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Circulation research Volume: 71 ISSN: 0009-7330 ISO Abbreviation: Circ. Res. Publication Date: 1992 Jul |
Date Detail:
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Created Date: 1992-07-23 Completed Date: 1992-07-23 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0047103 Medline TA: Circ Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 27-33 Citation Subset: IM |
Affiliation:
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Department of Medicine, Duke University Medical Center, Durham, N.C. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arteries Balloon Dilatation* Coronary Vessels* DNA / metabolism Dogs Femoral Artery / surgery Liposomes / metabolism Transfection* |
| Grant Support | |
ID/Acronym/Agency:
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P50-HL-17670-16/HL/NHLBI NIH HHS; R01-HL-26831/HL/NHLBI NIH HHS; R01-HL-37608-01/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Liposomes; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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