| Gene transfer in the nervous system and implications for transsynaptic neuronal tracing. | |
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MedLine Citation:
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PMID: 20367126 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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IMPORTANCE OF THE FIELD: Neuronal circuitries are determined by specific synaptic connections and they provide the cellular basis of cognitive processes and behavioral functions. To investigate neuronal circuitries, tracers are typically used to identify the original neurons and their projection targets. AREAS COVERED IN THIS REVIEW: Traditional tracing methods using chemical tracers have major limitations such as non-specificity. In this review, we highlight novel genetic tracing approaches that enable visualization of specific neuronal pathways by introducing cDNA encoding a transsynaptic tracer. In contrast to conventional tracing methods, these genetic approaches use cell-type-specific promoters to express transsynaptic tracers such as wheat germ agglutinin and C-terminal fragment of tetanus toxin, which allows labeling of either the input or output populations and connections of specific neuronal type. WHAT THE READER WILL GAIN: Specific neuronal circuit information by these genetic approaches will allow more precise, comprehensive and novel information about individual neural circuits and their function in normal and diseased brains. TAKE HOME MESSAGE: Using tracer gene transfer, neuronal circuit plasticity after traumatic injury or neurodegenerative diseases can be visualized. Also, this can provide a good marker for evaluation of therapeutic effects of neuroprotective or neurotrophic agents. |
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Authors:
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Youngbuhm Huh; Myung S Oh; Pierre Leblanc; Kwang-Soo Kim |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review |
Journal Detail:
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Title: Expert opinion on biological therapy Volume: 10 ISSN: 1744-7682 ISO Abbreviation: Expert Opin Biol Ther Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-13 Completed Date: 2010-07-12 Revised Date: 2012-04-09 |
Medline Journal Info:
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Nlm Unique ID: 101125414 Medline TA: Expert Opin Biol Ther Country: England |
Other Details:
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Languages: eng Pagination: 763-72 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Neurobiology, Kyung Hee University, Seoul, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Gene Transfer Techniques* Genetic Vectors Humans Neural Pathways / metabolism* Neuroanatomical Tract-Tracing Techniques / methods* Neuronal Tract-Tracers / metabolism* Peptide Fragments / biosynthesis, genetics Promoter Regions, Genetic Synapses / metabolism* Tetanus Toxin / biosynthesis, genetics Wheat Germ Agglutinins / biosynthesis, genetics |
| Grant Support | |
ID/Acronym/Agency:
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DC006501/DC/NIDCD NIH HHS; MH48866/MH/NIMH NIH HHS; R01 DC006501-05/DC/NIDCD NIH HHS; R01 MH048866-17/MH/NIMH NIH HHS; R01 MH048866-21/MH/NIMH NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Neuronal Tract-Tracers; 0/Peptide Fragments; 0/Tetanus Toxin; 0/Wheat Germ Agglutinins; 0/tetanus toxin fragment C |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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