Document Detail

Gene transfer effects on various cationic amphiphiles in CHO cells.
MedLine Citation:
PMID:  19002896     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Gene transfer is an important tool to explore genomic, cell biologic, or gene therapeutic research. In this paper we report that several cationic amphiphiles have the potential to efficiently deliver DNA into CHO cells, which is one of the cell lines considered to be important for production of proteins including therapeutic proteins. We have found that O,O'-ditetradecanoyl-N-(trimethylammonio acetyl) diethanolamine chloride (14Dea2), among 29 types of cationic amphiphiles tested, shows a transfection efficiency of more than 40% in CHO cells. In addition, the results from a series of hydrocarbon chains of varying lengths bound to a connector have shown that an optimal chain length is important for the efficient delivery of DNA into cells. Moreover, flow cytometer analysis has shown that 14Dea2 transfection leads to high levels of expression of the reporter gene (green fluorescent protein) in individual cells. These findings have suggested that 14Dea2 is able to effectively deliver a number of plasmids into a cell nucleus. Thus, our system might be a powerful tool for high efficiency gene transfer and production of high levels of recombinant protein.
Ken-Ichi Kusumoto; Tetsuyuki Akao; Eiichi Mizuki; Osamu Nakamura
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Publication Detail:
Type:  Journal Article     Date:  2006-09-07
Journal Detail:
Title:  Cytotechnology     Volume:  51     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2008-11-12     Completed Date:  2011-07-14     Revised Date:  2013-03-07    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  57-66     Citation Subset:  -    
Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, 1465-5 Aikawa Kurume, Fukuoka, 839-0861, Japan,
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