Document Detail


Gene transfer of antisense B7.1 attenuates acute rejection against splenic allografts in rats.
MedLine Citation:
PMID:  18089391     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Blockade of CD80-CD28 costimulatory pathway induces unresponsiveness of T cells to alloantigens and protects allografts against immune rejection in numerous animal models. The aim of this study was to investigate whether blocking expression of B7.1 (CD80) on donor splenocytes by an antisense technique protected splenic allografts against immune rejection. Splenic grafts from Wistar-Furth rats were intra-arterially transfused with an antisense B7.1 expression vector, before they were transplanted into Sprague-Dawley rats. The rats were sacrificed at scheduled times, and the splenic allografts histologically examined. Antisense gene transfer resulted in marked down-regulation of B7.1 in donor spleens, hyporesponsiveness of recipient T cells, and attenuated acute immune rejection against splenic allografts. No obvious damage to skin, liver, or gut due to graft-versus-host disease was detected in the recipients. In conclusion, blocking expression of B7.1 in donor spleens by antisense gene therapy represented a potential alloantigen-specific immunosuppressive strategy to inhibit acute rejection against splenic allografts.
Authors:
C Liu; S Pan; H Jiang; X Sun
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Transplantation proceedings     Volume:  39     ISSN:  0041-1345     ISO Abbreviation:  Transplant. Proc.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-19     Completed Date:  2008-02-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0243532     Medline TA:  Transplant Proc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3391-5     Citation Subset:  IM    
Affiliation:
Department of General Surgery, the Fourth Affiliated Hospital, and the First Clinical Medical School, Harbin Medical University, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, CD80 / genetics*
Antisense Elements (Genetics) / administration & dosage*
Gene Transfer Techniques
Graft Rejection / prevention & control*
Lymphocyte Culture Test, Mixed
Male
Plasmids
Rats
Rats, Inbred WF
Spleen / transplantation*
Transplantation, Homologous / immunology*
Chemical
Reg. No./Substance:
0/Antigens, CD80; 0/Antisense Elements (Genetics)

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