Document Detail


Gene therapy for human hepatocellular carcinoma with cytosine deaminase gene and prodrug flucytosine.
MedLine Citation:
PMID:  10678093     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate the antitumor effects of cytosine deaminase (CD) gene in combination with prodrug flucytosine (Flu, 5-fluorocytosine) on human hepatocellular carcinoma. METHODS: CD gene was transduced into human hepatocellular carcinoma cell line SMMC7721 with retroviral method and the cytotoxicity of Flu on the tumor cells was assayed in vitro with clonogenic techniques. The xenograft tumor model in nude mice was used to study in vivo therapeutic effects of CD gene/Flu system against human hepatocellular carcinoma. RESULTS: CD gene/Flu system had significant antitumor activities on human hepatocellular carcinoma cells in vitro and in nude mice. The antitumor activities of Flu 500 mg.kg-1 on hepatocellular carcinoma xenografts in nude mice were more potent than those of 5-fluouracil 10 mg.kg-1. CD gene/Flu system possessed bystander killing effects on hepatocellular carcinoma xenografts in nude mice. CONCLUSION: The experiment demonstrates the potential value of the CD gene/Flu system in the treatment of human hepatocellular carcinoma.
Authors:
H Z Lü; D Z Wu; Y L Wan; B Gu; H Z Gao; Y Q Liang; J X Wang
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zhongguo yao li xue bao = Acta pharmacologica Sinica     Volume:  20     ISSN:  0253-9756     ISO Abbreviation:  Zhongguo Yao Li Xue Bao     Publication Date:  1999 May 
Date Detail:
Created Date:  2000-05-18     Completed Date:  2000-05-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100330     Medline TA:  Zhongguo Yao Li Xue Bao     Country:  CHINA    
Other Details:
Languages:  eng     Pagination:  440-4     Citation Subset:  IM    
Affiliation:
Department of Clinical Pharmacology, Affiliated North Taiping Hospital, Chinese Academy of Medical Sciences, Beijing. luhz@usa.net
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / therapeutic use
Carcinoma, Hepatocellular / pathology,  therapy
Cytosine Deaminase
Female
Flucytosine / therapeutic use*
Gene Therapy*
Genes, Tumor Suppressor
Humans
Liver Neoplasms / pathology,  therapy*
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Transplantation
Nucleoside Deaminases / genetics*,  therapeutic use
Prodrugs / therapeutic use*
Transduction, Genetic
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Prodrugs; 2022-85-7/Flucytosine; EC 3.5.4.-/Nucleoside Deaminases; EC 3.5.4.1/Cytosine Deaminase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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