Document Detail

Gene Targeting and Subsequent Site-Specific Transgenesis at the β-actin (ACTB) Locus in Common Marmoset Embryonic Stem Cells.
MedLine Citation:
PMID:  21126169     Owner:  NLM     Status:  In-Data-Review    
Nonhuman primate embryonic stem (ES) cells have vast promise for preclinical studies. Genetic modification in nonhuman primate ES cells is an essential technique for maximizing the potential of these cells. The common marmoset (Callithrix jacchus), a nonhuman primate, is expected to be a useful transgenic model for preclinical studies. However, genetic modification in common marmoset ES (cmES) cells has not yet been adequately developed. To establish efficient and stable genetic modifications in cmES cells, we inserted the enhanced green fluorescent protein (EGFP) gene with heterotypic lox sites into the β-actin (ACTB) locus of the cmES cells using gene targeting. The resulting knock-in ES cells expressed EGFP ubiquitously under the control of the endogenous ACTB promoter. Using inserted heterotypic lox sites, we demonstrated Cre recombinase-mediated cassette exchange (RMCE) and successfully established a monomeric red fluorescent protein (mRFP) knock-in cmES cell line. Further, a herpes simplex virus-thymidine kinase (HSV-tk) knock-in cmES cell line was established using RMCE. The growth of tumor cells originating from the cell line was significantly suppressed by the administration of ganciclovir. Therefore, the HSV-tk/ganciclovir system is promising as a safeguard for stem cell therapy. The stable and ubiquitous expression of EGFP before RMCE enables cell fate to be tracked when the cells are transplanted into an animal. Moreover, the creation of a transgene acceptor locus for site-specific transgenesis will be a powerful tool, similar to the ROSA26 locus in mice.
Seiji Shiozawa; Kenji Kawai; Yohei Okada; Ikuo Tomioka; Takuji Maeda; Akifumi Kanda; Haruka Shinohara; Hiroshi Suemizu; Hirotaka James Okano; Yusuke Sotomaru; Erika Sasaki; Hideyuki Okano
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Publication Detail:
Type:  Journal Article     Date:  2011-01-16
Journal Detail:
Title:  Stem cells and development     Volume:  20     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-08-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1587-99     Citation Subset:  IM    
1 Department of Physiology, School of Medicine, Keio University , Tokyo, Japan .
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