| Gene structure, recombinant expression and functional characterization of grass carp leptin. | |
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MedLine Citation:
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PMID: 19857495 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Leptin is an important hormone for the regulation of food intake, energy expenditure and reproduction in mammals, but information regarding its role in teleosts remains scant. In the present study, the gene structures of grass carp (Ctenopharyngodon idellus) and silver carp (Hypophthalmichthys molitrix) leptins were characterized. Recombinant grass carp leptin (rgc-LEP) was expressed in Escherichia coli and purified, and identified by mass spectrometric analysis. A strong anorexic effect on food intake was observed in grass carp on the first day after intraperitoneal (IP) injection of rgc-LEP, but not during the following days. Body weight of the leptin group (LEP group) and the pair-fed group (PF group) showed no difference throughout the experimental period. The acute and chronic effects on the expression of key genes correlating to food intake, energy expenditure, lipid metabolism and digestion were further characterized by real-time PCR. Accordingly, the mRNA levels of neuropeptide Y (NPY), Stearoyl-CoA desaturase 1 (SCD1) and lipoprotein lipase (LPL) were significantly reduced whereas the mRNA levels of uncoupling protein 2 (UCP2), bile salt-activated lipase (BSAL) and fatty acid elongase (ELO) were significantly elevated on the first day after injection. No effect on the expression of these genes (except LPL) was observed on day 13. In contrast to the down-regulation by exogenous leptin in mammals, the mRNA level of grass carp leptin was elevated 5.76-fold on the first day after rgc-LEP treatment. Our results suggest that leptin has an acute effect on the regulation of food intake, energy expenditure and lipid metabolism in grass carp, but the effect can be rapidly counteracted through mechanisms that are currently unknown. |
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Authors:
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Guan-Gui Li; Xu-Fang Liang; Qiuling Xie; Guangzhao Li; Ying Yu; Kaaseng Lai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-24 |
Journal Detail:
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Title: General and comparative endocrinology Volume: 166 ISSN: 1095-6840 ISO Abbreviation: Gen. Comp. Endocrinol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-17 Completed Date: 2010-08-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370735 Medline TA: Gen Comp Endocrinol Country: United States |
Other Details:
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Languages: eng Pagination: 117-27 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Inc. All rights reserved. |
Affiliation:
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College of Life Science and Technology, Jinan University, Guangzhou 510632, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetyltransferases
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genetics Animals Body Weight / drug effects Carps* Eating / drug effects, genetics Energy Metabolism / drug effects, genetics Fish Proteins / genetics*, metabolism Gene Expression Regulation Ion Channels / genetics Leptin / genetics*, metabolism, pharmacology Lipid Metabolism / drug effects, genetics Lipoprotein Lipase / genetics Mitochondrial Proteins / genetics Neuropeptide Y / genetics Recombinant Proteins / metabolism, pharmacology Stearoyl-CoA Desaturase / genetics Sterol Esterase / genetics |
| Chemical | |
Reg. No./Substance:
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0/Fish Proteins; 0/Ion Channels; 0/Leptin; 0/Mitochondrial Proteins; 0/Neuropeptide Y; 0/Recombinant Proteins; 0/mitochondrial uncoupling protein 2; EC 1.14.19.1/Stearoyl-CoA Desaturase; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/fatty acid elongases; EC 3.1.1.-/bile salt-stimulated lipase; EC 3.1.1.13/Sterol Esterase; EC 3.1.1.34/Lipoprotein Lipase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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