| Gene structure for the alpha 1 chain of a human short-chain collagen (type XIII) with alternatively spliced transcripts and translation termination codon at the 5' end of the last exon. | |
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MedLine Citation:
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PMID: 2459707 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two overlapping human genomic clones that encode a short-chain collagen, designated alpha 1(XIII), were isolated by using recently described cDNA clones. Characterization of the cosmid clones that span approximately equal to 65,000 base pairs (bp) of the 3' end of the gene established several unusual features of this collagen gene. The last exon encodes solely the 3' untranslated region and it begins with a complete stop codon. The 10 adjacent exons vary in size from 27 to 87 bp and two of them are 54 bp. Therefore, the alpha 1-chain gene of type XIII collagen has some features found in genes for fibrillar collagens but other features that are distinctly different. Previous analysis of overlapping cDNA clones and nuclease S1 mapping of mRNAs indicated one alternative splicing site causing a deletion of 36 bp from the mature mRNA. The present study showed that the 36 bp is contained within the gene as a single exon and also that the gene has a 45-bp -Gly-Xaa-Xaa- repeat coding exon not found in the cDNA clones previously characterized. Nuclease S1 mapping experiments indicated that this 45-bp exon is found in normal human skin fibroblast mRNAs. Accordingly, the data demonstrate that there is alternative splicing of at least two exons of the type alpha 1(XIII)-chain gene. |
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Authors:
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L Tikka; T Pihlajaniemi; P Henttu; D J Prockop; K Tryggvason |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 85 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1988 Oct |
Date Detail:
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Created Date: 1988-11-21 Completed Date: 1988-11-21 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7491-5 Citation Subset: IM |
Affiliation:
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Biocenter, University of Oulu, Finland. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/J04085; M20795; M20796; M20797; M20798; M20799; M20800; M20801; M20802; M20803; M20804; M20805 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Cloning, Molecular Codon / genetics* Collagen / genetics* DNA / genetics Exons* Humans Introns Molecular Sequence Data Protein Biosynthesis RNA / genetics RNA Splicing RNA, Messenger / genetics* Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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AR-38188/AR/NIAMS NIH HHS; AR-38923/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Codon; 0/RNA, Messenger; 63231-63-0/RNA; 9007-34-5/Collagen; 9007-49-2/DNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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