Document Detail


Gene-specific characterization of human histone H2B by electron capture dissociation.
MedLine Citation:
PMID:  16457587     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The basis set of protein forms expressed by human cells from the H2B gene family was determined by Top Down Mass Spectrometry. Using Electron Capture Dissociation for MS/MS of H2B isoforms, direct evidence for the expression of unmodified H2B.Q, H2B.A, H2B.K/T, H2B.J, H2B.E, H2B.B, H2B.F, and monoacetylated H2B.A was obtained from asynchronous HeLa cells. H2B.A was the most abundant form, with the overall expression profile not changing significantly in cells arrested in mitosis by colchicine or during mid-S, mid-G2, G2/M, and mid-G1 phases of the cell cycle. Modest hyperacetylation of H2B family members was observed after sodium butyrate treatment.
Authors:
Nertila Siuti; Michael J Roth; Craig A Mizzen; Neil L Kelleher; James J Pesavento
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of proteome research     Volume:  5     ISSN:  1535-3893     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-02-06     Completed Date:  2006-03-29     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  233-9     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetylation / drug effects
Amino Acid Sequence
Butyrates / pharmacology
Cell Cycle / drug effects*
Colchicine / toxicity
Hela Cells
Histones / chemistry*
Humans
Mass Spectrometry
Molecular Sequence Data
Protein Isoforms / chemistry
Grant Support
ID/Acronym/Agency:
5T32 GM 08276/GM/NIGMS NIH HHS; GM 067193-03/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Butyrates; 0/Histones; 0/Protein Isoforms; 64-86-8/Colchicine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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