Document Detail

Gene silencing using the recessive rice bacterial blight resistance gene xa13 as a new paradigm in plant breeding.
MedLine Citation:
PMID:  22218673     Owner:  NLM     Status:  Publisher    
Resistant germplasm resources are valuable for developing resistant varieties in agricultural production. However, recessive resistance genes are usually overlooked in hybrid breeding. Compared with dominant traits, however, they may confer resistance to different pathogenic races or pest biotypes with different mechanisms of action. The recessive rice bacterial blight resistance gene xa13, also involved in pollen development, has been cloned and its resistance mechanism has been recently characterized. This report describes the conversion of bacterial blight resistance mediated by the recessive xa13 gene into a dominant trait to facilitate its use in a breeding program. This was achieved by knockdown of the corresponding dominant allele Xa13 in transgenic rice using recently developed artificial microRNA technology. Tissue-specific promoters were used to exclude most of the expression of artificial microRNA in the anther to ensure that Xa13 functioned normally during pollen development. A battery of highly bacterial blight resistant transgenic plants with normal seed setting rates were acquired, indicating that highly specific gene silencing had been achieved. Our success with xa13 provides a paradigm that can be adapted to other recessive resistance genes.
Changyan Li; Jing Wei; Yongjun Lin; Hao Chen
Related Documents :
1850083 - Interaction of abrb, a transcriptional regulator from bacillus subtilis with the promot...
24205013 - Microarray analysis of tomato's early and late wound response reveals new regulatory ta...
18628293 - Processing of triplex-directed psoralen dna interstrand crosslinks by recombination mec...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-5
Journal Detail:
Title:  Plant cell reports     Volume:  -     ISSN:  1432-203X     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9880970     Medline TA:  Plant Cell Rep     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Thermo-sensitive imprinted polymer coating CdTe quantum dots for target protein specific recognition...
Next Document:  The use of plants for the production of therapeutic human peptides.