Document Detail

Gene printer: laser-scanning targeted transfection of cultured cardiac neonatal rat cells.
MedLine Citation:
PMID:  16916749     Owner:  NLM     Status:  MEDLINE    
Heterogeneous gene expression in cardiac cells and tissues which requires targeted delivery of foreign DNA into selected cells or regions is needed for the development of novel therapies. Several techniques have been employed for targeted transfection, such as direct microinjection into cells or targeted electroporation. However, these techniques have limited bandwidth or spatial resolution of transfection. We aimed to develop a method for transfection of cardiac cells by means of laser-assisted optoporation using a standard confocal microscope. This technique allows for the transfection of selected cell types in the presence of other cell types as long as they are distinguishable with a microscope. This technique can work as a "gene printer" creating arbitrarily shaped areas of transfected cells.
Alena V Nikolskaya; Vladimir P Nikolski; Igor R Efimov
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Cell communication & adhesion     Volume:  13     ISSN:  1541-9061     ISO Abbreviation:  Cell Commun. Adhes.     Publication Date:    2006 Jul-Aug
Date Detail:
Created Date:  2006-08-18     Completed Date:  2006-11-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101096596     Medline TA:  Cell Commun Adhes     Country:  United States    
Other Details:
Languages:  eng     Pagination:  217-22     Citation Subset:  IM    
Department of Biomedical Engineering, Washington University, St. Louis, MO 63005, USA.
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MeSH Terms
Animals, Newborn
Cell Culture Techniques
Cells, Cultured
Feasibility Studies
Gene Targeting / methods*
Lipids / pharmacology
Microscopy, Confocal / methods*
Myocardium / cytology*
Myocytes, Cardiac / cytology
Research Design
Transfection / methods*
Grant Support
Reg. No./Substance:
0/Lipids; 0/Lipofectamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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