Document Detail


Gene organization around the phenylalanyl-transfer ribonucleic acid synthetase locus in Escherichia coli.
MedLine Citation:
PMID:  7012115     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The organization of seven genes located at about 38 min on the genetic map of Escherichia coli was examined; these genes included pheS and pheT, which code for the alpha and beta subunits of phenylalanyl-transfer ribonucleic acid synthetase, and thrS, the structural gene for threonyl-transfer ribonucleic acid synthetase. Deletion mutants were isolated from an F-prime-containing merodiploid strain and were characterized genetically. Seventeen different kinds of deletions extending into pheS of pheT were identified. These deletions unambiguously defined the gene order as aroD pps himA pheT pheS thrS pfkB. Mutants with deletions covering either pheS or pheT, but not both, were analyzed further by assay of phenylalanyl-transfer ribonucleic acid synthetase. The phenotype of the mutants with a deletion from pfkB through pheS was anomalous; although the pheT gene was apparently still present, its product, the beta subunit, was much reduced in activity.
Authors:
M M Comer
Related Documents :
16025755 - Sequence learning: response structure and effector transfer.
24723775 - The millipede typhloglomeriscaucasica golovatch, 1975 found epigeically (diplopoda, glo...
20504315 - Transcriptome analysis of the mobile genome iceclc in pseudomonas knackmussii b13.
23357025 - Interleukin-17 in pearl oyster (pinctada fucata): molecular cloning and functional char...
8127885 - Details of the evolutionary history from invertebrates to vertebrates, as deduced from ...
21213595 - Phylogenetic and evolutionary analysis of vibrio parahaemolyticus and vibrio alginolyti...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  146     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1981 Apr 
Date Detail:
Created Date:  1981-06-25     Completed Date:  1981-06-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  269-74     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acyl-tRNA Synthetases / genetics*
Chromosome Mapping
Escherichia coli / enzymology,  genetics*
Mutation
Phenylalanine-tRNA Ligase / genetics*,  metabolism
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
GM24774/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
EC 6.1.1.-/Amino Acyl-tRNA Synthetases; EC 6.1.1.20/Phenylalanine-tRNA Ligase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Properties of a mutant Escherichia coli phosphoenolpyruvate carboxylase deficient in coregulation by...
Next Document:  Short deoxyribonucleic acid repair patch length in Escherichia coli is determined by the processive ...