Document Detail


Gene localization, size, and physical map of the chromosome of Streptococcus pneumoniae.
MedLine Citation:
PMID:  1657889     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A physical map of the Streptococcus (Diplococcus) pneumoniae chromosome, which is circular and 2,270 kbp in circumference, has been constructed. The restriction enzymes ApaI, SmaI, and SacII were used to digest intact chromosomes, and the fragments were resolved by field inversion gel electrophoresis (FIGE). The digests produced 22, 20, and 29 fragments, respectively. The order of the fragments was deduced from Southern blot hybridization of isolated labeled fragments to separated fragments of the various restriction digests. Genetic markers were correlated with the physical map by transformation of recipient cells with FIGE-isolated DNA fragments derived from genetically marked S. pneumoniae strains. In addition, markers were mapped by the hybridization of cloned genes to FIGE-separated restriction fragments. Six rRNA gene (rrn) clusters were mapped by hybridization to rrn-containing fragments of Haemophilus influenzae.
Authors:
A M Gasc; L Kauc; P Barraillé; M Sicard; S Goodgal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  173     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1991 Nov 
Date Detail:
Created Date:  1991-12-20     Completed Date:  1991-12-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7361-7     Citation Subset:  IM    
Affiliation:
Centre de Recherche de Biochimie et de Génétique Cellulaires, Centre National de la Recherche Scientifique, Toulouse, France.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosomes, Bacterial*
DNA Restriction Enzymes / metabolism
DNA, Bacterial / genetics,  isolation & purification
DNA, Circular / genetics
Deoxyribonucleases, Type II Site-Specific / metabolism*
Genetic Markers
Restriction Mapping*
Streptococcus pneumoniae / genetics*
Substrate Specificity
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Circular; 0/Genetic Markers; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.4/CCCGGG-specific type II deoxyribonucleases; EC 3.1.21.4/CCGCGG-specific type II deoxyribonucleases; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific; EC 3.1.21.4/GGGCCC-specific type II deoxyribonucleases
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