Document Detail

Gene expressions associated with chemosensitivity in human hepatoma cells.
MedLine Citation:
PMID:  17523305     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: Only limited patients with hepatoma benefit from chemotherapy without a clear explanation. We aimed to identify genes associated with chemosensitivity using transcriptional profiles. METHODOLOGY: In 8 hepatoma cells (HLE, HLF, Huh7, Hep3B, PLC/PRF/5, SK-Hep1, Huh6, and HepG2) transcriptional profiles were obtained using cDNA microarray including 2300 genes. Chemosensitivities to 8 anticancer drugs (nimustine, mitomycin C, cisplatin, carboplatin, doxorubicin, epirubicin, mitoxantrone, and 5-fluorouracil) were measured by obtaining 50% growth inhibitory concentrations (GI50) using MTT assay. Genes having drug-specific association with chemosensitivity were selected. RESULTS: Up-regulation of topoisomerase II beta was associated with chemo-resistance, the target of doxorubicin. Platinum-specific resistance was associated with superoxide dismutase 2 expression. Antigen peptide transporter 1 expression correlated with nimustine and mitoxantrone-specific susceptibility. These results were verified by semi-quantitative RT-PCR. Drug inactivators reported in non-liver cancers such as multidrug transporters and drug metabolizers showed less diversity of chemosensitivity in hepatoma cells. CONCLUSIONS: To evaluate these gene expressions may be useful to select anticancer drugs, and possibly to consider new therapeutic target to modify drug action.
Yujin Hoshida; Masaru Moriyama; Motoyuki Otsuka; Naoya Kato; Hiroyoshi Taniguchi; Yasushi Shiratori; Naohiko Seki; Masao Omata
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Hepato-gastroenterology     Volume:  54     ISSN:  0172-6390     ISO Abbreviation:  Hepatogastroenterology     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-05-25     Completed Date:  2007-08-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8007849     Medline TA:  Hepatogastroenterology     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  489-92     Citation Subset:  IM    
Department of Gastroenterology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.
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MeSH Terms
Antigens, Neoplasm / genetics
Antineoplastic Agents / pharmacology*
Carcinoma, Hepatocellular / genetics*,  pathology*
Cell Division / drug effects*
Cell Line, Tumor
Cell Survival / drug effects*
DNA Topoisomerases, Type II, Eukaryotic / genetics
DNA-Binding Proteins / genetics
Dose-Response Relationship, Drug
Drug Resistance, Neoplasm / genetics*
Gene Expression Profiling*
Gene Expression Regulation, Neoplastic / genetics
Liver Neoplasms / genetics*,  pathology*
Membrane Transport Proteins / genetics
Oligonucleotide Array Sequence Analysis
Reverse Transcriptase Polymerase Chain Reaction
Superoxide Dismutase / genetics
Up-Regulation / genetics
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Antineoplastic Agents; 0/DNA-Binding Proteins; 0/Membrane Transport Proteins; 97599-47-8/peptide permease; EC Dismutase; EC 5.99.1.-/DNA Topoisomerases, Type II, Eukaryotic; EC topoisomerase II alpha

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