Document Detail

Gene expression profiling of skin and draining lymph nodes of rats affected with cutaneous contact hypersensitivity.
MedLine Citation:
PMID:  16977379     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES AND DESIGN: The present study aimed at a broad genome expression analysis of rat skin and draining lymph nodes affected with allergic contact dermatitis (ACD). METHODS: ACD was elicited in sensitized SD rats with 0.5% 2,4-dinitrofluorobenzene on skin areas distant from the sensitization sites. Involved skin and lymph nodes were dissected 8 and 24 h after challenge. RNA was hybridized on Affymetrix GeneChips. Expression data were analyzed with the GeneSpring software. RESULTS: Expression of 1,882 out of 8,799 examined genes in skin or lymph nodes was significantly (p < or = 0.01) changed compared to levels in normal tissue. After an additional 2- fold filtering, four expression patterns were selected and interpreted. Prominently up-regulated genes were IL-6, CCR-5, CCL-2, CCL-3, CXCL-1, CXCL-10, TIMP-1, OX-40, calgranulin b, ST2, beta-defensin, iNOS, STAT-1, MMP-3, MMP-9, MMP-12, and MMP-13. CONCLUSIONS: In addition to previously reported transcript changes, the present findings suggest/support that plasma cells, mast cells, and a specific IFN-gamma pathway play a substantial role in the pathogenesis of ACD.
B Hartmann; F Staedtler; N Hartmann; J Meingassner; H Firat
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Inflammation research : official journal of the European Histamine Research Society ... [et al.]     Volume:  55     ISSN:  1023-3830     ISO Abbreviation:  Inflamm. Res.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-09-15     Completed Date:  2006-12-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9508160     Medline TA:  Inflamm Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  322-34     Citation Subset:  IM    
Novartis Institutes of Biomedical Research ATDA, Brunnerstrasse 59, 1230, Vienna, Austria.
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MeSH Terms
DNA Primers / chemistry
Dermatitis, Contact / pathology*
Dinitrofluorobenzene / pharmacology
Gene Expression Profiling*
Lymph Nodes / pathology*
Nucleic Acid Hybridization
Principal Component Analysis
RNA / metabolism
Skin / drug effects,  pathology
Skin Diseases / metabolism,  pathology*
Reg. No./Substance:
0/DNA Primers; 63231-63-0/RNA; 70-34-8/Dinitrofluorobenzene

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