Document Detail

Gene expression profiling of human cardiac potassium and sodium channels.
MedLine Citation:
PMID:  16257073     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The native cardiac ion currents and the action potential itself are the results of the concerted action of several different ion channels. The electrophysiological properties of cardiac cells are determined by the composition of ion channels and by their absolute abundance and proportional ratio. METHODS: Our aim in this study was to compare the gene expression level of a representative panel of cardiac ion channels with each other and to compare the same channels in the atrium and ventricle of the human heart using quantitative real-time PCR analysis. RESULTS: We obtained a significant difference in the gene expression levels in 21 of 35 channels between atrium and ventricle of healthy human hearts. Further, we found that the expression levels of Kv1.5 and Kv2.1 transcripts in the ventricle were very high, and that mRNAs for Kv1.7 and Kv3.4 are highly abundant in both the atrium and ventricle, which might indicate a functional role of these ion channel subunits in the formation of action potential in the human ventricle and both in the atrium and ventricle, respectively. CONCLUSIONS: This is the first report on the expression of several ion channel subunits, such as Kv1.7, Kv3.3 or Kv3.4 in human cardiomyocytes. The expression levels of these genes are comparable with that of well known ion channel subunits. Therefore, it is reasonable to assume, that these ion channel subunits may contribute to native currents in the human myocardium.
Balázs Ordög; Endre Brutyó; László G Puskás; Julius G Papp; András Varró; János Szabad; Zsolt Boldogkoi
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-27
Journal Detail:
Title:  International journal of cardiology     Volume:  111     ISSN:  0167-5273     ISO Abbreviation:  Int. J. Cardiol.     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-08-18     Completed Date:  2007-01-03     Revised Date:  2008-10-28    
Medline Journal Info:
Nlm Unique ID:  8200291     Medline TA:  Int J Cardiol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  386-93     Citation Subset:  IM    
Department of Biology, Faculty of Medicine, University of Szeged, Somogyi B. 4. H-6720, Szeged, Hungary.
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MeSH Terms
Ether-A-Go-Go Potassium Channels / metabolism
Gene Expression Profiling*
Heart Atria / metabolism*
Heart Ventricles / metabolism*
Kv1.5 Potassium Channel / metabolism
Polymerase Chain Reaction
Potassium Channels / metabolism*
Potassium Channels, Tandem Pore Domain / metabolism
Potassium Channels, Voltage-Gated / metabolism
RNA, Messenger / metabolism
Shab Potassium Channels / metabolism
Shaw Potassium Channels / metabolism
Sodium Channels / metabolism*
Reg. No./Substance:
0/ERG1 potassium channel; 0/Ether-A-Go-Go Potassium Channels; 0/KCNC4 protein, human; 0/KCNE1 protein, human; 0/KCNK1 protein, human; 0/Kv1.5 Potassium Channel; 0/Potassium Channels; 0/Potassium Channels, Tandem Pore Domain; 0/Potassium Channels, Voltage-Gated; 0/RNA, Messenger; 0/Shab Potassium Channels; 0/Shaw Potassium Channels; 0/Sodium Channels

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