Document Detail


Gene expression profiling of granulosa cells from PCOS patients following varying doses of human chorionic gonadotropin.
MedLine Citation:
PMID:  23381551     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Human chorionic gonadotrophin (hCG) has been used to induce ovulation and oocyte maturation. Although the most common dose of hCG used in IVF is 10,000 IU, there are reports that suggest 5,000 IU is sufficient to yield similar results. The objective of this study is to evaluate the dose dependent differences in gene expression of granulosa cells following various doses of hCG treatment.
METHODS: Patients with polycystic ovarian syndrome (PCOS) were stimulated for IVF treatment. The hCG injection was either withheld or given at 5,000 or 10,000 IU. Granulosa cells from the follicular fluids have been collected for RNA isolation and analyzed using Affymetrix genechip arrays.
RESULTS: Unsupervised hierarchical clustering based on whole gene expression revealed two distinct groups of patients in this experiment. All untreated patients were clustered together whereas hCG-treated patients separated to a different group regardless of the dose. A large number of the transcripts were similarly up- or down-regulated across both hCG doses (2229 and 1945 transcripts, respectively). However, we observed dose-dependent statistically significant differences in gene expression in only 15 transcripts.
CONCLUSIONS: Although hCG injection caused a major change in the gene expression profile of granulosa cells, 10,000 IU hCG resulted in minimal changes in the gene expression profiles of granulosa cells as compared with 5,000 IU. Thus, based on our results, we suggest the use of 10,000 IU hCG should be reconsidered in PCOS patients.
Authors:
Serdar Coskun; Hasan H Otu; Khalid A Awartani; Laila A Al-Alwan; Saad Al-Hassan; Hend Al-Mayman; Namik Kaya; Mehmet S Inan
Publication Detail:
Type:  Journal Article     Date:  2013-02-05
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  30     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-26     Completed Date:  2013-09-23     Revised Date:  2014-03-06    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  341-52     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Chorionic Gonadotropin / administration & dosage*
Dose-Response Relationship, Drug
Female
Fertilization in Vitro*
Gene Expression Regulation / drug effects
Granulosa Cells / metabolism
Humans
Microarray Analysis
Middle Aged
Oocytes* / drug effects,  growth & development
Ovulation / drug effects
Ovulation Induction
Polycystic Ovary Syndrome / drug therapy,  pathology*
Pregnancy
Pregnancy Rate
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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