Document Detail


Gene expression profiles of vitrified in vitro- and in vivo-derived bovine blastocysts.
MedLine Citation:
PMID:  22778065     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Vitrification is becoming a preferred method for pre-implantation embryo cryopreservation. The objective of this study was to determine the differentially expressed genes of in vivo- and in vitro-produced bovine embryos after vitrification. In vitro- (IVF) and in vivo-derived (IVV) bovine blastocysts were identified as follows: in vitro-produced fresh (IVF F), in vitro-produced vitrified (IVF V), in vivo-derived fresh (IVV F), in vivo-derived vitrified (IVV V). The microarray results showed that 53 genes were differentially regulated between IVF and IVV, and 121 genes were differentially regulated between fresh and vitrified blastocysts (P < 0.05). There were 6, 268, 962 and 17 differentially regulated genes between IVF-F x IVV-F; IVF-V x IVV-V; IVF-F x IVF-V; and IVV-F x IVV-V, respectively (P < 0.05). While gene expression was significantly different between fresh and vitrified IVF blastocysts (P < 0.05), it was similar between fresh and vitrified IVV blastocysts. Significantly up-regulated KEGG pathways included ribosome, oxidative phosphorylation, spliceosome, and oocyte meiosis in the fresh, while sphingolipid and purine metabolisms were up-regulated in the vitrified IVF blastocyst. The results showed that in vitro bovine blastocyst production protocols used in this study caused no major gene expression differences compared to those of in vivo-produced blastocysts. After vitrification, however, in vitro-produced blastocysts showed major gene expression differences compared to in vivo blastocysts. This study suggests that in vitro-produced embryos are of comparable quality to their in vivo counterparts. Vitrification of in vitro blastocysts, on the other hand, causes significant up-regulation of genes that are involved in stress responses. Mol. Reprod. Dev. © 2012 Wiley Periodicals, Inc.
Authors:
Digdem Aktoprakligil Aksu; Cansu Agca; Soner Aksu; Haydar Bagis; Tolga Akkoc; Arzu Tas Caputcu; Sezen Arat; Ali Cihan Taskin; Sedat H Kizil; Tahir Karasahin; Numan Akyol; Muharrem Satilmis; Hakan Sagirkaya; Burcu Ustuner; Zekeriya Nur; Yuksel Agca
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-9
Journal Detail:
Title:  Molecular reproduction and development     Volume:  -     ISSN:  1098-2795     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
TUBITAK MRC Genetic Engineering and Biotechnology Institute, Gebze, Turkey.
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