Document Detail


Gene expression profiles of adipose tissue of obese rats after central administration of neuropeptide Y-Y5 receptor antisense oligodeoxynucleotides by cDNA microarrays.
MedLine Citation:
PMID:  18652865     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the gene expression profiles of adipose tissue of obese rats after central administration of neuropeptide Y-Y5 receptor antisense oligodeoxynucleotides (ODNs), Y5 receptor antisense, mismatched ODNs or vehicle was intracerebroventricularly injected and cDNA microarrays were undertaken. Central administration of NPY-Y5 receptor antisense ODNs decreased food intake, body weight and serum insulin compared with both vehicle and mismatched ODNs. The average area of adipocytes both at retroperitoneal and epididymal adipose tissue were fall in antisense group while only the weight of the retroperitoneal fat pats was reduced in antisense group. cDNA microarrays containing 18,000 genes/Ests were used to investigate gene expression of adipose tissue. Autoradiographic analysis showed that 404, 81, and 34 genes were differently expressed over twofold, threefold, and fivefold, respectively. The analysis of gene expression profiles indicated that 332 genes were up-regulated and 187 genes were down-regulated in response to Y5 receptor antisense ODNs treatment. Different clusters of genes associated with apoptosis, signal transduction, energy metabolism, lipid metabolism, etc., such as FXR1, PHLDA1, MAEA, PIK3R1, ICAM2, PITPN, CALM2, CAMK2D, PKIA, DRD2, SLC25A14, CKB, AADAC, LIPA, ACOX3, FADS1, were concerned. Analysis of differentially expressed genes will help to understand the effects of Y5 receptor antisense ODNs therapy.
Authors:
Jie Qiu; Yu-Hui Ni; Rong-Hua Chen; Chen-Bo Ji; Feng Liu; Chun-Mei Zhang; Chun-Lin Gao; Xiao-Hui Chen; Mei-Ling Tong; Xia Chi; Xiao-Yu Zhou; Xi-Rong Guo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-04
Journal Detail:
Title:  Peptides     Volume:  29     ISSN:  0196-9781     ISO Abbreviation:  Peptides     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-22     Completed Date:  2009-04-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2052-60     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, Nanjing Maternity and Child Health Hospital of Nanjing Medical University, 210004 Nanjing, China; Institute of Pediatrics of Nanjing Medical University, 210029 Nanjing, China.
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MeSH Terms
Descriptor/Qualifier:
Adipocytes, White / drug effects
Adipose Tissue / metabolism*
Animals
Eating / drug effects
Gene Expression Profiling
Insulin / blood
Male
Oligonucleotide Array Sequence Analysis
Oligonucleotides, Antisense / pharmacology
Rats
Rats, Sprague-Dawley
Receptors, Neuropeptide Y / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Oligonucleotides, Antisense; 0/Receptors, Neuropeptide Y; 0/neuropeptide Y5 receptor; 11061-68-0/Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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