Document Detail


Gene expression, ontogeny and transplacental induction of hepatic UDP-glucuronosyl transferase activity in mice.
MedLine Citation:
PMID:  1914788     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The ontogeny and transplacental inducibility of UDP-glucuronosyl transferase (UDPGT) activities potentially relevant to detoxification of polycyclic aromatic hydrocarbons were studied in (C57BL/6 x DBA/2) F1 or (DBA/2 x C57BL/6) F1 fetal mouse liver, with p-nitrophenol (PNP) and 3-hydroxybenzo[a]pyrene (3-OH-BP) as substrates. Both UDPGT activities developed during the late fetal period and reached almost 60% of the adult activity at term; PNP, but not 3-OH-BP UDPGT decreased significantly on postnatal day 1 before rising to adult levels. A single exposure to beta-naphthoflavone (beta NF; 150 mg/kg) on day 17 of gestation induced the PNP-UDPGT activity significantly (1.5-fold) by day 19 in the B6D2 F1s but not D2B6 F1s. A single dose of 3-methylcholanthrene (MC; 100 mg/kg) or 2,3,7,8,-tetrachlorodibenzo-p-dioxin (10 micrograms/kg) did not induce, but three injections of MC also resulted in significant induction in the fetuses of C57BL/6 mothers. 3-OH-BP-UDPGT was not significantly induced by any of the chemicals in either genetic cross. In a parallel study, a gene for an inducible mouse UDPGT, designated UDPGTm-1, was shown by Northern blotting to be expressed in fetal liver by day 18 of gestation at low levels relative to adults, but was not induced transplacentally by MC, beta NF or phenobarbital (PB). These results show that (1) at least two functionally defined UDPGT activities toward phenolic substrates are present in the late fetal mouse liver; (2) one of these is transplacentally inducible by beta NF and MC, but only in fetuses of C57BL/6 mothers, (3) induction where achieved was relatively small in magnitude, and (4) a gene of a PB-inducible UDPGT was expressed at low levels in the fetuses but was not induced transplacentally.
Authors:
D P Chauhan; M S Miller; I S Owens; L M Anderson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Developmental pharmacology and therapeutics     Volume:  16     ISSN:  0379-8305     ISO Abbreviation:  Dev Pharmacol Ther     Publication Date:  1991  
Date Detail:
Created Date:  1991-11-01     Completed Date:  1991-11-01     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8003947     Medline TA:  Dev Pharmacol Ther     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  139-49     Citation Subset:  IM    
Affiliation:
Laboratory of Comparative Carcinogenesis, National Cancer Institute, Frederick Cancer Research Facility, Md.
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MeSH Terms
Descriptor/Qualifier:
Animals
Benzoflavones / pharmacology
Enzyme Induction
Fetus / enzymology*
Gene Expression
Gestational Age
Glucuronosyltransferase / biosynthesis*
Liver / embryology,  enzymology*
Methylcholanthrene / pharmacology
Mice
Mice, Inbred C57BL / embryology
Mice, Inbred DBA / embryology
Mice, Inbred Strains / embryology
Tetrachlorodibenzodioxin / pharmacology
beta-Naphthoflavone
Chemical
Reg. No./Substance:
0/Benzoflavones; 1746-01-6/Tetrachlorodibenzodioxin; 56-49-5/Methylcholanthrene; 6051-87-2/beta-Naphthoflavone; EC 2.4.1.17/Glucuronosyltransferase

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