| Gene expression and inducibility of the aryl hydrocarbon receptor-dependent pathway in cultured bovine blood lymphocytes. | |
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MedLine Citation:
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PMID: 21803134 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The exposure to dioxin-like (DL) compounds, an important class of persistent environmental pollutants, results in the altered expression of target genes. This occurs through the binding to the aryl hydrocarbon receptor (AhR), the subsequent dimerization with the AhR nuclear translocator (ARNT), and the binding of the complex to DNA responsive elements. A number of genes are up-regulated, including, among others, the AhR repressor (AHRR) and several biotransformation enzymes, such as the members of CYP1 family and NAD(P)H-quinone oxidoreductase (NOQ1). The expression and the inducibility of the above genes were investigated in mitogen-stimulated cultured blood lymphocytes from cattle, which represent a notable source of DL-compound human exposure through dairy products and meat. As assessed by real-time PCR, all the examined genes except CYP1A2 and NQO1 were detected under basal conditions. Cell exposure to the DL-compounds PCB126 or PCB77 in the 10(-6)-10(-9)M concentration range resulted in a 2-4-fold induction of CYPIA1 and CYP1B1, which was antagonized by α-naphthoflavone or PCB153. This study demonstrates for the first time the presence and inducibility of the AhR pathway in easily accessible cells like bovine peripheral lymphocytes and prompts further investigations to verify whether similar changes could occur under in vivo conditions. |
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Authors:
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Flavia Girolami; Veronica Spalenza; Monica Carletti; Giovanni Perona; Paola Sacchi; Roberto Rasero; Carlo Nebbia |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-07-23 |
Journal Detail:
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Title: Toxicology letters Volume: 206 ISSN: 1879-3169 ISO Abbreviation: Toxicol. Lett. Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-08-29 Completed Date: 2011-10-21 Revised Date: 2012-01-24 |
Medline Journal Info:
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Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: Netherlands |
Other Details:
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Languages: eng Pagination: 204-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Animal Pathology, University of Turin, Via Leonardo da Vinci 44, Grugliasco, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aryl Hydrocarbon Hydroxylases / antagonists & inhibitors, genetics, metabolism Benzoflavones / pharmacology Cattle Cells, Cultured Cytochrome P-450 CYP1A1 / genetics, metabolism Environmental Pollutants / pharmacology* Enzyme Induction / drug effects Enzyme Inhibitors / pharmacology Gene Expression Regulation / drug effects* Lymphocyte Activation / drug effects Lymphocytes / drug effects*, metabolism* NAD(P)H Dehydrogenase (Quinone) / genetics, metabolism Polychlorinated Biphenyls / pharmacology* RNA, Messenger / metabolism Receptors, Aryl Hydrocarbon / agonists, antagonists & inhibitors, biosynthesis*, genetics Repressor Proteins / genetics, metabolism Signal Transduction / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Benzoflavones; 0/Environmental Pollutants; 0/Enzyme Inhibitors; 0/Polychlorinated Biphenyls; 0/RNA, Messenger; 0/Receptors, Aryl Hydrocarbon; 0/Repressor Proteins; 32598-13-3/3,4,3',4'-tetrachlorobiphenyl; 35065-27-1/2,4,5,2',4',5'-hexachlorobiphenyl; 57465-28-8/3,4,5,3',4'-pentachlorobiphenyl; 604-59-1/alpha-naphthoflavone; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.14.14.1/Cytochrome P-450 CYP1A1; EC 1.14.14.1/cytochrome P450, family 1, subfamily B, polypeptide 1; EC 1.6.5.2/NAD(P)H Dehydrogenase (Quinone) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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