| Gene expression in hepatocyte-like lines established by targeted carcinogenesis in transgenic mice. | |
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MedLine Citation:
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PMID: 1373387 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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New hepatocyte-like cell lines (mhAT) were derived from the liver of a transgenic mouse expressing SV40 early genes under the direction of the liver-specific antithrombin III gene promoter (ATIII-TSV40). Their differentiated phenotypes were improved and stabilized by the use of liver-specific growth media (arginine-free, glucose-free, or low-fructose/glucose-free medium). The best differentiated lines display a very high level of albumin, transferrin, and L-type pyruvate kinase (L-PK) gene expression that is comparable to that observed in the mouse liver. Abundance of the aldolase B and phosphoenolpyruvate carboxykinase (PEPCK) transcripts varied from 5 to 35% of the in vivo concentrations while abundance of the alpha-fetoprotein and phenylalanine hydroxylase transcripts remained very low. Hormonal (cAMP and insulin) and nutritional (glucose) gene controls of PEPCK and L-PK were, at least partially, conserved. mhAT cells are readily transfectable by the calcium phosphate coprecipitation technique and exhibit a liver-specific pattern of expression of exogenous genes. Thus, mhAT cells seem suitable for the analysis of the regulatory regions involved in the tissue-specific transcription of genes. This work demonstrates, therefore, the great efficiency of targeted carcinogenesis in transgenic mice to create new differentiated cell lines. The availability of various lines of liver-specific cells with different phenotypes will constitute useful tools to establish correlations between expression of trans-acting factors and control of the phenotype. |
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Authors:
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B Antoine; F Levrat; V Vallet; T Berbar; N Cartier; N Dubois; P Briand; A Kahn |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 200 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1992 May |
Date Detail:
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Created Date: 1992-05-18 Completed Date: 1992-05-18 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 175-85 Citation Subset: IM |
Affiliation:
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Institut Cochin de Génétique Moléculaire, laboratoire de recherche en génétique et pathologie moléculaires (INSERM U129), Paris, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Albumins
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analysis Animals Antigens, Viral, Tumor / genetics Cell Line / metabolism* Gene Expression Regulation* Liver / metabolism* Mice Mice, Transgenic Phenotype Phosphoenolpyruvate Carboxykinase (GTP) / genetics Promoter Regions, Genetic Pyruvate Kinase / genetics RNA, Messenger / analysis Trans-Activators Transfection Transferrin / analysis Tumor Cells, Cultured alpha-Fetoproteins / analysis |
| Chemical | |
Reg. No./Substance:
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0/Albumins; 0/Antigens, Viral, Tumor; 0/RNA, Messenger; 0/Trans-Activators; 0/alpha-Fetoproteins; 11096-37-0/Transferrin; EC 2.7.1.40/Pyruvate Kinase; EC 4.1.1.32/Phosphoenolpyruvate Carboxykinase (GTP) |
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