| Gene expression in endothelial cells and intimal smooth muscle cells in atherosclerosis-prone or atherosclerosis-resistant regions of the human aorta. | |
| | |
MedLine Citation:
|
PMID: 18212511 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BACKGROUND/AIMS: We compared the atherogenic gene expression in the intimas of atherosclerosis-prone regions (proximal walls), which are exposed to disturbed shear stress, and atherosclerosis-resistant regions (apices), which are exposed to unidirectional laminar shear stress, at the orifices of the intercostal arteries of human aortas. METHODS AND RESULTS: Expression of mRNAs, detected by in situ RT-PCR, for IL-1 beta, TNF-alpha, VCAM-1, PAF receptor and GRP in endothelial cells (ECs), and of PDGF receptor beta (PDGFR-beta), MCP-1, GRP and collagen type-1 by smooth muscle cells (SMCs) in the proximal walls, was significantly enhanced, while seldom observed in the elastic-hyperplastic layer of the apices. Protein expression of PDGFR-beta, IL-1 beta and TNF-alpha was also observed on the proximal walls. SMC growth in the apices was inhibited. Cultured SMC growth and their expression of PDGFR-beta were also significantly inhibited by elastin. CONCLUSION: These results suggest that the construction of the elastic-hyperplastic layer and the subsequent inhibition of SMC growth by elastin, with stabilized ECs under unidirectional laminar shear stress, resulted in atherosclerosis-resistant regions at the apices of human aortas, and that the continuous induction of atherogenic gene expression by ECs activated by disturbed shear stress inhibits the formation of atherosclerosis-resistant intima along the proximal walls. |
| | |
Authors:
|
A K M Khyrul Wara; Masako Mitsumata; Tetsu Yamane; Yoshiaki Kusumi; Yoji Yoshida |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-22 |
Journal Detail:
|
Title: Journal of vascular research Volume: 45 ISSN: 1423-0135 ISO Abbreviation: J. Vasc. Res. Publication Date: 2008 |
Date Detail:
|
Created Date: 2008-06-04 Completed Date: 2008-07-23 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9206092 Medline TA: J Vasc Res Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 303-13 Citation Subset: IM |
Copyright Information:
|
(c) 2008 S. Karger AG, Basel. |
Affiliation:
|
Cardiology Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Aorta / cytology*, metabolism Atherosclerosis / etiology* Elastin / physiology Endothelial Cells / metabolism* Endothelium, Vascular Female Gene Expression Profiling* Humans Male Middle Aged Muscle, Smooth, Vascular Myocytes, Smooth Muscle / metabolism* RNA, Messenger / analysis Stress, Mechanical |
| Chemical | |
Reg. No./Substance:
|
0/RNA, Messenger; 9007-58-3/Elastin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Adaptation of hindquarter vascular reactivity to femoral artery ligation and hypercholesterolemia in...
Next Document: Homocysteinemia correlates with plasma thiol redox status in patients with end-stage renal disease.