Document Detail

Gene expression in endothelial cells and intimal smooth muscle cells in atherosclerosis-prone or atherosclerosis-resistant regions of the human aorta.
MedLine Citation:
PMID:  18212511     Owner:  NLM     Status:  MEDLINE    
BACKGROUND/AIMS: We compared the atherogenic gene expression in the intimas of atherosclerosis-prone regions (proximal walls), which are exposed to disturbed shear stress, and atherosclerosis-resistant regions (apices), which are exposed to unidirectional laminar shear stress, at the orifices of the intercostal arteries of human aortas. METHODS AND RESULTS: Expression of mRNAs, detected by in situ RT-PCR, for IL-1 beta, TNF-alpha, VCAM-1, PAF receptor and GRP in endothelial cells (ECs), and of PDGF receptor beta (PDGFR-beta), MCP-1, GRP and collagen type-1 by smooth muscle cells (SMCs) in the proximal walls, was significantly enhanced, while seldom observed in the elastic-hyperplastic layer of the apices. Protein expression of PDGFR-beta, IL-1 beta and TNF-alpha was also observed on the proximal walls. SMC growth in the apices was inhibited. Cultured SMC growth and their expression of PDGFR-beta were also significantly inhibited by elastin. CONCLUSION: These results suggest that the construction of the elastic-hyperplastic layer and the subsequent inhibition of SMC growth by elastin, with stabilized ECs under unidirectional laminar shear stress, resulted in atherosclerosis-resistant regions at the apices of human aortas, and that the continuous induction of atherogenic gene expression by ECs activated by disturbed shear stress inhibits the formation of atherosclerosis-resistant intima along the proximal walls.
A K M Khyrul Wara; Masako Mitsumata; Tetsu Yamane; Yoshiaki Kusumi; Yoji Yoshida
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-01-22
Journal Detail:
Title:  Journal of vascular research     Volume:  45     ISSN:  1423-0135     ISO Abbreviation:  J. Vasc. Res.     Publication Date:  2008  
Date Detail:
Created Date:  2008-06-04     Completed Date:  2008-07-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9206092     Medline TA:  J Vasc Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  303-13     Citation Subset:  IM    
Copyright Information:
(c) 2008 S. Karger AG, Basel.
Cardiology Division, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
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MeSH Terms
Aorta / cytology*,  metabolism
Atherosclerosis / etiology*
Elastin / physiology
Endothelial Cells / metabolism*
Endothelium, Vascular
Gene Expression Profiling*
Middle Aged
Muscle, Smooth, Vascular
Myocytes, Smooth Muscle / metabolism*
RNA, Messenger / analysis
Stress, Mechanical
Reg. No./Substance:
0/RNA, Messenger; 9007-58-3/Elastin

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