Document Detail


Gene expression of human squamous cell carcinoma antigens 1 and 2 in human cell lines.
MedLine Citation:
PMID:  11182054     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The squamous cell carcinoma antigen (SCCA) serves as a serological marker for squamous cell carcinomas. Molecular cloning of the SCCA genomic region has revealed the presence of two tandemly arrayed genes, SCCA1 and SCCA2. SCCA1 is a papain-like cysteine proteinase inhibitor, while SCCA2 is a chymotrypsin-like serine proteinase inhibitor. Little is known concerning how expression of the SCCA1 and SCCA2 genes is regulated in human cell lines. The purpose of this study was to determine whether the SCCA1 gene or SCCA2 gene is more strongly expressed in human cell lines. Squamous cell carcinoma cell lines secreted respectively 4 times and 50 times as much SCCA proteins into medium as normal human keratinocyte and non-squamous cell carcinoma cell lines, as measured by enzyme-linked immunosorbent assay. Quantitative RT-PCR ELISA digoxigenin-labeling assay demonstrated that SCCA1 mRNA expression in squamous cell carcinoma cell lines was respectively 2.8 times and 42 times that in keratinocyte and non-squamous cell carcinoma cell lines. The ratio of SCCA1 to SCCA2 mRNA expression differed distinctly among squamous cell carcinoma, keratinocyte and non-squamous cell carcinoma cell lines (2.8, squamous; 24.1, keratinocyte; 11.0, non-squamous). These findings suggest that SCCA1 is mainly expressed in squamous cell carcinoma, keratinocyte and non-squamous cell carcinoma cell lines and that the ratio of SCCA1 to SCCA2 expression might be a novel marker for the detection of squamous cell carcinoma.
Authors:
K Hamada; Y Hanakawa; K Hashimoto; M Iwamoto; T Kihana; S Hirose; M Nakamura; M Ito
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Oncology reports     Volume:  8     ISSN:  1021-335X     ISO Abbreviation:  Oncol. Rep.     Publication Date:    2001 Mar-Apr
Date Detail:
Created Date:  2001-02-22     Completed Date:  2001-04-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9422756     Medline TA:  Oncol Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  347-54     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, School of Medicine, Ehime University, Shitsukawa, Ehime 791-0295, Japan. hamakatu@m.ehime-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / pathology
Antigens, Neoplasm / analysis,  genetics*
Carcinoma, Squamous Cell / genetics*,  pathology
Enzyme-Linked Immunosorbent Assay
Female
Humans
Keratinocytes / cytology
Male
Reverse Transcriptase Polymerase Chain Reaction
Serpins*
Tumor Cells, Cultured
Tumor Markers, Biological / analysis,  genetics*
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Serpins; 0/Tumor Markers, Biological; 0/squamous cell carcinoma-related antigen

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