| Gene expression of human sperm component related to A4 amyloid precursor protein. | |
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MedLine Citation:
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PMID: 1550421 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Monoclonal antibodies were raised against a specific human sperm protein and designated as the YWK-II mAb. The partial cDNA encoding the protein was isolated from a rat testis lambda gt11 expression library and the amino acid sequence of the protein was deduced. The cytoplasmic-transmembrane domains of the deduced protein had high homology with the A4 amyloid precursor protein of Alzheimer's disease. To evaluate the stage of spermatogenesis when the gene was expressed, single-stranded 35S-labeled RNA probes were prepared from the cDNA. By an in situ hybridization technique the mRNA for the antigen was detected in germ cells at all stages of spermatogenesis. The finding that the gene is expressed in spermatogonia suggests possible involvement in the initiation of germ cell differentiation or in the detachment of spermatogonia from the basement membrane. |
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Authors:
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Y C Yan; S Y Miao; C Zong; Y H Li; L F Wang; S S Koide |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Archives of andrology Volume: 28 ISSN: 0148-5016 ISO Abbreviation: Arch. Androl. Publication Date: 1992 Jan-Feb |
Date Detail:
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Created Date: 1992-04-21 Completed Date: 1992-04-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7806755 Medline TA: Arch Androl Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1-6 Citation Subset: IM |
Affiliation:
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Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amyloid beta-Protein
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analysis,
genetics*,
metabolism Antibodies, Monoclonal / immunology Autoradiography DNA / genetics Gene Expression / genetics* Humans Male Nucleic Acid Hybridization Protein Precursors / analysis, genetics*, metabolism RNA Probes RNA, Messenger / genetics Spermatogenesis / genetics Spermatozoa / chemistry*, metabolism Sulfur Radioisotopes |
| Chemical | |
Reg. No./Substance:
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0/Amyloid beta-Protein; 0/Antibodies, Monoclonal; 0/Protein Precursors; 0/RNA Probes; 0/RNA, Messenger; 0/Sulfur Radioisotopes; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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