Document Detail

Gene expression of human sperm component related to A4 amyloid precursor protein.
MedLine Citation:
PMID:  1550421     Owner:  NLM     Status:  MEDLINE    
Monoclonal antibodies were raised against a specific human sperm protein and designated as the YWK-II mAb. The partial cDNA encoding the protein was isolated from a rat testis lambda gt11 expression library and the amino acid sequence of the protein was deduced. The cytoplasmic-transmembrane domains of the deduced protein had high homology with the A4 amyloid precursor protein of Alzheimer's disease. To evaluate the stage of spermatogenesis when the gene was expressed, single-stranded 35S-labeled RNA probes were prepared from the cDNA. By an in situ hybridization technique the mRNA for the antigen was detected in germ cells at all stages of spermatogenesis. The finding that the gene is expressed in spermatogonia suggests possible involvement in the initiation of germ cell differentiation or in the detachment of spermatogonia from the basement membrane.
Y C Yan; S Y Miao; C Zong; Y H Li; L F Wang; S S Koide
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of andrology     Volume:  28     ISSN:  0148-5016     ISO Abbreviation:  Arch. Androl.     Publication Date:    1992 Jan-Feb
Date Detail:
Created Date:  1992-04-21     Completed Date:  1992-04-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7806755     Medline TA:  Arch Androl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1-6     Citation Subset:  IM    
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing.
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MeSH Terms
Amyloid beta-Protein / analysis,  genetics*,  metabolism
Antibodies, Monoclonal / immunology
DNA / genetics
Gene Expression / genetics*
Nucleic Acid Hybridization
Protein Precursors / analysis,  genetics*,  metabolism
RNA Probes
RNA, Messenger / genetics
Spermatogenesis / genetics
Spermatozoa / chemistry*,  metabolism
Sulfur Radioisotopes
Reg. No./Substance:
0/Amyloid beta-Protein; 0/Antibodies, Monoclonal; 0/Protein Precursors; 0/RNA Probes; 0/RNA, Messenger; 0/Sulfur Radioisotopes; 9007-49-2/DNA

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