| Gene expression of flap endonuclease-1 during cell proliferation and differentiation. | |
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MedLine Citation:
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PMID: 10771101 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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It has been shown that flap endonuclease-1 (FEN-1), a structure-specific nuclease, acts on the removal of RNA primers during Okazaki fragment maturation in DNA synthesis. To study whether the gene expression of FEN-1 is inducible during cell proliferation, we analyzed the FEN-1 mRNA levels in actively growing cells and non-growing cells. The gene expression of FEN-1 was higher in mitotic cells than in resting cells, and was markedly decreased, especially, when terminal differentiation was induced in promyelocytic leukemia cells (HL-60 cells). The decline correlated substantially with the ceasing of DNA synthesis. In the examination of tissue-specific gene expression, the human testis, spleen, thymus and mucosal lining of colon tissues expressed this gene actively, whereas the prostate, ovary, small intestine and peripheral blood leukocyte hardly expressed it. In addition, FEN-1 was co-localized with the proliferating cell nuclear antigen (PCNA) in young rat kidney according to immunohistochemistry. These findings suggest that FEN-1 gene expression is inducible during cell proliferation for DNA synthesis, and is down-regulated during cell differentiation. |
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Authors:
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I S Kim; M Y Lee; I H Lee; S L Shin; S Y Lee |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1496 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2000 Apr |
Date Detail:
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Created Date: 2000-06-29 Completed Date: 2000-06-29 Revised Date: 2012-06-01 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 333-40 Citation Subset: IM |
Affiliation:
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Department of Natural Sciences, Chemistry Section, College of Medicine, The Catholic University of Korea, 505 Banpo-Dong, Socho-Gu, Seoul, South Korea. ikim@cmc.cuk.ac.kr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation Cell Division DNA / analysis, biosynthesis DNA Replication* Endodeoxyribonucleases / biosynthesis, genetics* Flap Endonucleases Gene Expression Regulation, Enzymologic Granulocytes / cytology HL-60 Cells Humans Immunohistochemistry Kidney / metabolism Lymphocyte Activation Lymphocytes / cytology Male Proliferating Cell Nuclear Antigen RNA, Messenger / analysis Rats Reverse Transcriptase Polymerase Chain Reaction Spleen / metabolism Testis / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Proliferating Cell Nuclear Antigen; 0/RNA, Messenger; 9007-49-2/DNA; EC 3.1.-/Endodeoxyribonucleases; EC 3.1.-/Flap Endonucleases; EC 3.1.11.-/FEN1 protein, human |
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