Document Detail

Gene expression changes in the secondary palate and mandible of Prdm16(-/-) mice.
MedLine Citation:
PMID:  23149718     Owner:  NLM     Status:  MEDLINE    
Loss of Prdm16 expression in the mouse leads to a complete cleft of the secondary palate. We have now determined changes in gene expression in the secondary palates of Prdm16(-/-) fetuses in an attempt to reveal the mechanism(s) leading to the failure of palate closure in these mice. Defined pathway-based polymerase chain reaction arrays were used to analyze the expression of genes associated with the extracellular matrix and the transforming growth factor-β and bone morphogenetic protein signaling networks, perturbations of which can lead to palatal clefting. Loss of Prdm16 expression in the secondary palate leads to alterations in numerous genes within these groups, many of which have been linked to chondrogenesis and osteogenesis. The expression of several genes linked to bone development was significantly changed in the developing secondary palate. Analysis of gene expression in the mandibles of Prdm16(-/-) fetuses revealed similar alterations in the same gene set. These data suggest that one function of Prdm16 is the regulation of genes that play a role in the differentiation of mesenchymal cells into chondro-/osteocytes.
Dennis R Warner; Justin P Wells; Robert M Greene; M Michele Pisano
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-11-13
Journal Detail:
Title:  Cell and tissue research     Volume:  351     ISSN:  1432-0878     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-27     Completed Date:  2013-08-07     Revised Date:  2014-03-07    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  445-52     Citation Subset:  IM    
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MeSH Terms
Blotting, Western
Bone Morphogenetic Proteins / genetics,  metabolism
Chondrogenesis / genetics
DNA-Binding Proteins / deficiency*,  genetics,  metabolism*
Extracellular Matrix Proteins / genetics,  metabolism
Fetus / metabolism
Gene Expression Regulation, Developmental*
Mandible / embryology,  metabolism*
Mice, Knockout
Osteogenesis / genetics
Osteopontin / metabolism
Palate / embryology,  metabolism*
Signal Transduction / genetics
Transcription Factors / deficiency*,  genetics,  metabolism*
Transforming Growth Factor beta / genetics,  metabolism
Grant Support
Reg. No./Substance:
0/Bone Morphogenetic Proteins; 0/DNA-Binding Proteins; 0/Extracellular Matrix Proteins; 0/Prdm16 protein, mouse; 0/Transcription Factors; 0/Transforming Growth Factor beta; 106441-73-0/Osteopontin

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