| Gene expression of brain natriuretic peptide in isolated atrial and ventricular human myocardium: influence of angiotensin II and diastolic fiber length. | |
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MedLine Citation:
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PMID: 11120697 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: We studied the effects of angiotensin II (Ang II) and diastolic overstretch on the induction of cardiac growth in isometrically contracting muscle preparations from human right atria and left ventricles. We used the gene expression of brain natriuretic peptide (BNP) as a molecular marker of cardiac hypertrophy. METHODS AND RESULTS: Northern blot analysis was performed in human atrial muscle preparations, which were either incubated in 10(-6) mol/L Ang II for 45 minutes or diastolically stretched to 120% of optimum muscle length. Similar experiments were performed with human left ventricular muscle preparations. Results were as follows: (1) BNP gene expression increased in human atrial myocardium 4-fold when stimulated by Ang II (n=7, P<0.001). (2) Diastolic overstretch increased BNP expression in a time-dependent manner. The linear regression equations for the BNP/GAPDH ratio as a function of time (hours) were y=1.21+0.62x (P:<0.001) for overstretched preparations and y=1.07-0.01x (P:=NS) for atrial preparations kept at physiological muscle length. (3) In left ventricular human muscle preparations, diastolic overstretch and Ang II increased BNP gene expression as well. (4) In addition, the Ang II subtype 1 receptor blocker losartan was able to block the effects of Ang II and diastolic overstretch. CONCLUSIONS: Cardiac hypertrophy can be induced in isolated human atrial and left ventricular intact myocardium by Ang II and diastolic overstretch but not by isometric afterload. The fact that the induction of cardiac growth is inhibited by the blockade of Ang II subtype 1 receptors is of scientific and clinical importance. |
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Authors:
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S Wiese; T Breyer; A Dragu; R Wakili; T Burkard; S Schmidt-Schweda; E M Füchtbauer; U Dohrmann; F Beyersdorf; D Radicke; C J Holubarsch |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Circulation Volume: 102 ISSN: 1524-4539 ISO Abbreviation: Circulation Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2000-12-26 Completed Date: 2001-01-04 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0147763 Medline TA: Circulation Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3074-9 Citation Subset: IM |
Affiliation:
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Department of Cardiology, Medizinische Universitätsklinik, and the Department of Cardiac Surgery, University of Freiburg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Angiotensin II
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pharmacology,
physiology* Blotting, Northern Cardiomegaly / pathology*, physiopathology Diastole Heart Atria / metabolism Heart Ventricles / metabolism Humans Isometric Contraction Muscle Fibers, Skeletal / pathology* Myocardial Contraction Myocardium / metabolism*, pathology*, ultrastructure Natriuretic Peptide, Brain / metabolism* RNA, Messenger / metabolism Stress, Mechanical |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 11128-99-7/Angiotensin II; 114471-18-0/Natriuretic Peptide, Brain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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