Document Detail


Gene expression analysis: Teaching Students to Do 30,000 Experiments at once with Microarray.
MedLine Citation:
PMID:  22615226     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Genome scale experiments routinely produce large data sets that require computational analysis, yet there are few student-based labs that illustrate the design and execution of these experiments. In order for students to understand and participate in the genomic world, teaching labs must be available where students generate and analyze large data sets. We present a microarray-based gene expression analysis experiment that is tailored for undergraduate students. The methods in this article describe an expression analysis experiment that can also be applied to CGH and SNP experiments. Factors such as technical difficulty, duration, cost, and availability of materials and equipments are considered in the lab design. The microarray teaching lab is performed in two sessions. The first is an introductory wet bench exercise that allows students to master the basic technical skills. The second builds on the concepts and skills with students acquiring and analyzing the microarray data. This lab exercise familiarizes students with large-scale data experiments and introduces them to the initial analysis steps.
Authors:
Felicia I Carvalho; Christopher Johns; Marc E Gillespie
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Publication Detail:
Type:  Journal Article     Date:  2012-02-15
Journal Detail:
Title:  Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology     Volume:  40     ISSN:  1539-3429     ISO Abbreviation:  Biochem Mol Biol Educ     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100970605     Medline TA:  Biochem Mol Biol Educ     Country:  United States    
Other Details:
Languages:  eng     Pagination:  181-90     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley Periodicals, Inc.
Affiliation:
Department of Pharmaceutical Sciences, St Johns University, New York. carvalhf@stjohns.edu.
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