Document Detail


Gene expression of P2X-receptors in the developing inner ear of the rat.
MedLine Citation:
PMID:  10505627     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reverse transcription-polymerase chain reaction (RT-PCR) was used to characterize the expression of P2X receptor subunits (P2X1-P2X7) in different inner ear tissues. The present study revealed the presence of P2X2, P2X3, P2X4 and P2X7-mRNA in rat organ of Corti, vestibular organ and spiral ganglion at different postnatal developmental stages (PD1-PD16), with slight differences in the onset of expression. Expression of P2X1, P2X5 and P2X6-mRNA was not detectable in the inner ear tissues. In addition, single cell RT-PCR experiments with outer hair cells (OHC) revealed the expression of either the P2X2 or the P2X2-2 splice variant or coexpression of both isoforms in individual cells. Our data suggest that extracellular adenosine-5'-triphosphate (ATP) may play an important role in signal transduction in the inner ear.
Authors:
U Brändle; H P Zenner; J P Ruppersberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  273     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-22     Completed Date:  1999-11-22     Revised Date:  2010-01-14    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  105-8     Citation Subset:  IM; S    
Affiliation:
Department of Otolaryngology, University of Tuebingen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Aging / metabolism
Animals
Animals, Newborn / growth & development,  metabolism
DNA, Recombinant
Ear, Inner / physiology*
Gene Expression*
Genetic Variation
Hair Cells, Auditory, Outer / metabolism
Organ of Corti / metabolism
Protein Isoforms / genetics
RNA, Messenger / metabolism
Rats
Rats, Wistar
Receptors, Purinergic P2 / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Spiral Ganglion / metabolism
Vestibule, Labyrinth / metabolism
Chemical
Reg. No./Substance:
0/DNA, Recombinant; 0/Protein Isoforms; 0/RNA, Messenger; 0/Receptors, Purinergic P2; 0/purinergic P2X2 receptor

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