| Gene expression of P2X-receptors in the developing inner ear of the rat. | |
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MedLine Citation:
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PMID: 10505627 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Reverse transcription-polymerase chain reaction (RT-PCR) was used to characterize the expression of P2X receptor subunits (P2X1-P2X7) in different inner ear tissues. The present study revealed the presence of P2X2, P2X3, P2X4 and P2X7-mRNA in rat organ of Corti, vestibular organ and spiral ganglion at different postnatal developmental stages (PD1-PD16), with slight differences in the onset of expression. Expression of P2X1, P2X5 and P2X6-mRNA was not detectable in the inner ear tissues. In addition, single cell RT-PCR experiments with outer hair cells (OHC) revealed the expression of either the P2X2 or the P2X2-2 splice variant or coexpression of both isoforms in individual cells. Our data suggest that extracellular adenosine-5'-triphosphate (ATP) may play an important role in signal transduction in the inner ear. |
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Authors:
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U Brändle; H P Zenner; J P Ruppersberg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neuroscience letters Volume: 273 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 1999 Oct |
Date Detail:
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Created Date: 1999-11-22 Completed Date: 1999-11-22 Revised Date: 2010-01-14 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: IRELAND |
Other Details:
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Languages: eng Pagination: 105-8 Citation Subset: IM; S |
Affiliation:
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Department of Otolaryngology, University of Tuebingen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aging
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metabolism Animals Animals, Newborn / growth & development, metabolism DNA, Recombinant Ear, Inner / physiology* Gene Expression* Genetic Variation Hair Cells, Auditory, Outer / metabolism Organ of Corti / metabolism Protein Isoforms / genetics RNA, Messenger / metabolism Rats Rats, Wistar Receptors, Purinergic P2 / genetics* Reverse Transcriptase Polymerase Chain Reaction Spiral Ganglion / metabolism Vestibule, Labyrinth / metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA, Recombinant; 0/Protein Isoforms; 0/RNA, Messenger; 0/Receptors, Purinergic P2; 0/purinergic P2X2 receptor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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