| Gene expression of 5-fluorouracil metabolic enzymes in hepatocellular carcinoma and non-tumor tissue. | |
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MedLine Citation:
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PMID: 18230555 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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5-fluorouracil (5-FU) is a basic agent used in chemotherapy. The aim of this study is to investigate the gene expression of 5-FU anabolic and catabolic enzymes in hepatocellular carcinoma (HCC) and non-tumor tissue, respectively to increase our knowledge of resistant mechanisms to 5-FU in HCC. The relative mRNA level of orotate phosphoribosyltransferase (OPRT), ribonucleotide reductase (RNR), dihydropyrimidine dehydrogenase (DPD) and target enzyme thymidylate synthase (TS), were analyzed in 30 matched samples of HCC (T) and non-tumor tissue (NT) using quantitative RT-PCR. The expression of OPRT, RNR-M1, RNR-M2 and TS is significantly higher in T compared with in NT (1.3-fold increase, 1.6-fold, 7.1-fold, 1.9-fold, respectively), but that of DPD showed no difference between T and NT. Our results show that HCC should not be treated with 5-FU alone because of its instability in liver. |
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Authors:
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M Sunaga; T Tomonaga; M Yoshikawa; M Ebara; H Shimada; H Saisho; F Nomura |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of chemotherapy (Florence, Italy) Volume: 19 ISSN: 1120-009X ISO Abbreviation: J Chemother Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2008-01-30 Completed Date: 2008-07-15 Revised Date: 2009-08-04 |
Medline Journal Info:
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Nlm Unique ID: 8907348 Medline TA: J Chemother Country: Italy |
Other Details:
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Languages: eng Pagination: 709-15 Citation Subset: IM |
Affiliation:
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Department of Molecular Diagnosis, Chiba University, Japan. sunaga-m@unim.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Hepatocellular
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enzymology* Dihydrouracil Dehydrogenase (NADP) / metabolism Female Fluorouracil / metabolism* Gene Expression Humans Liver / enzymology* Liver Neoplasms / enzymology* Male Middle Aged Orotate Phosphoribosyltransferase / metabolism Ribonucleotide Reductases / metabolism Thymidylate Synthase / metabolism |
| Chemical | |
Reg. No./Substance:
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51-21-8/Fluorouracil; EC 1.17.4.-/Ribonucleotide Reductases; EC 1.3.1.2/Dihydrouracil Dehydrogenase (NADP); EC 2.1.1.45/Thymidylate Synthase; EC 2.4.2.10/Orotate Phosphoribosyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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