Document Detail

Gene dosage for 5S ribosomal ribonucleic acid in Escherichia coli ad Bacillus megaterium.
MedLine Citation:
PMID:  4993318     Owner:  NLM     Status:  MEDLINE    
The number of gene copies for 5S ribosomal ribonucleic acid (rRNA), relative to that for 16 and 23S rRNA, has been determined by deoxyribonucleic acid (DNA)-RNA hybridization for Escherichia coli and Bacillus megaterium. In both cases, the number of 5S rRNA genes equals the number of 16 or 23S rRNA genes. Rapid procedures for preparing extremely highly purified DNA suitable for DNA-RNA hybridization experiments and chemically pure 5S rRNA are described.
B Pace; N R Pace
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  105     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1971 Jan 
Date Detail:
Created Date:  1971-03-20     Completed Date:  1971-03-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  142-9     Citation Subset:  IM    
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MeSH Terms
Bacillus megaterium*
Centrifugation, Density Gradient
Chromatography, DEAE-Cellulose
DNA, Bacterial / isolation & purification
Reg. No./Substance:
0/Acrylates; 0/DNA, Bacterial; 64-17-5/Ethanol

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