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Gene delivery of cyclin-dependent kinase inhibitors p21 ( Waf1 ) and p27 ( Kip1 ) suppresses proliferation of MCF-7 breast cancer cells in vitro.
MedLine Citation:
PMID:  23338153     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Because tumorigenesis depends on a variety of oncogenes, symphyseal study of combined genes may lead to more significant knowledge about tumorigenesis and progression. Combined deficiency of p21 and p27 proteins in mice is linked to more aggressive spontaneous tumorigenesis. We investigated the effect of the transfected p21 ( Waf1 )-p27 ( Kip1 ) gene on centrosome duplication, cell proliferation, and apoptosis of MCF-7, a breast cancer cell line. METHODS: The pIRES-p21 ( Waf1 ), pIRES-p27 ( Kip1 ), and pIRES-p21 ( Waf1 )-p27 ( Kip1 ) genes were transfected into MCF-7 cells by lipofection. The effect on proliferation was evaluated by MTT assay and clone-formation assay. Cell cycle and apoptosis were analyzed by flow cytometry. Apoptosis was tested by flow cytometry and TUNEL assay. Centrosome duplication was detected by use of indirect immunofluorescence microscopy. RESULTS: The results showed that the pIRES-p21 ( Waf1 ), pIRES-p27 ( Kip1 ), and pIRES-p21 ( Waf1 )-p27 ( Kip1 ) significantly inhibited proliferation of MCF-7 cells, followed by accumulation of MCF-7 cells in cycle G(1), induced apoptosis, and a decrease in the proportion of MCF-7 cells which contained abnormal centrosomes. Compared with p21 ( Waf1 ) or p27 ( Kip1 ) alone, combination of p21 ( Waf1 ) and p27 ( Kip1 ) had a much more significant effect (P < 0.05). CONCLUSION: Altogether, these results indicate that the p21 ( Waf1 )-p27 ( Kip1 ) gene combination has a more obvious antitumor effect than p21 ( Waf1 ) or p27 ( Kip1 ) alone. This study provides preclinical evidence that combination of p21 ( Waf1 ) and p27 ( Kip1 ) could be a novel and promising therapeutic approach to treatment of breast cancer with suppressed p21 ( Waf1 ) and p27 ( Kip1 ) expression.
Authors:
Dandan Jiang; Xingang Wang; Xiangping Liu; Funian Li
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-22
Journal Detail:
Title:  Breast cancer (Tokyo, Japan)     Volume:  -     ISSN:  1880-4233     ISO Abbreviation:  Breast Cancer     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888201     Medline TA:  Breast Cancer     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Breast Surgery, The Affiliated Hospital of Medical College, Qingdao University, Qingdao, 266003, Shandong, China.
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