Document Detail


Gene characterized for membrane desaturase that produces (E)-11 isomers of mono- and diunsaturated fatty acids.
MedLine Citation:
PMID:  11805319     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Moth species have evolved integral membrane desaturases that exhibit a wide diversity in substrate specificity, as well as in regiospecificity and stereospecificity of the unsaturated products. We report here the cloning and expression of a single desaturase from the sex pheromone gland of the light brown apple moth, Epiphyas postvittana, that makes E11 isomers of monounsaturated (E11-16 and E11-14) fatty acids and a diunsaturated (E9,E11-14) fatty acid. In the pheromone gland, the monoene precursor is made available by beta oxidation of E11-16 acid with a subsequent two-carbon loss to E9-14 acid. A functional assay using a baculovirus expression system required addition of myristic acid and E9-14 acid precursors to demonstrate the unusual regiospecificity and stereospecificity of this desaturase. The amino acid sequence of this desaturase has approximately 61% identity to that of Z11-desaturases from two other insect species, and only approximately 48% identity to the metabolic Z9-desaturases in those species. A pheromone-gland Z9-desaturase gene also was found with the light brown apple moth that differed in its deduced amino acid sequence (66% identity) with the metabolic Z9-desaturase from fat body in this species.
Authors:
Weitian Liu; Hongmei Jiao; Nancy C Murray; Marion O'Connor; Wendell L Roelofs
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  99     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2002 Jan 
Date Detail:
Created Date:  2002-01-23     Completed Date:  2002-04-29     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  620-4     Citation Subset:  IM    
Affiliation:
Department of Entomology, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AF402775;  AY049741;  AY061988
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Baculoviridae / genetics
Base Sequence
Cell Line
Cloning, Molecular
DNA, Complementary / genetics
Fat Body / enzymology
Fatty Acid Desaturases / genetics*,  metabolism*
Fatty Acids, Unsaturated / biosynthesis,  chemistry
Female
Gene Expression
Genes, Insect*
Molecular Sequence Data
Moths / enzymology*,  genetics*
Recombinant Proteins / genetics,  metabolism
Sequence Homology, Amino Acid
Sex Attractants / biosynthesis
Stereoisomerism
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Fatty Acids, Unsaturated; 0/Recombinant Proteins; 0/Sex Attractants; EC 1.14.19.-/Fatty Acid Desaturases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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