Document Detail

Gene loops enhance transcriptional directionality.
MedLine Citation:
PMID:  23019609     Owner:  NLM     Status:  MEDLINE    
Eukaryotic genomes are extensively transcribed, forming both messenger RNAs (mRNAs) and noncoding RNAs (ncRNAs). ncRNAs made by RNA polymerase II often initiate from bidirectional promoters (nucleosome-depleted chromatin) that synthesize mRNA and ncRNA in opposite directions. We demonstrate that, by adopting a gene-loop conformation, actively transcribed mRNA encoding genes restrict divergent transcription of ncRNAs. Because gene-loop formation depends on a protein factor (Ssu72) that coassociates with both the promoter and the terminator, the inactivation of Ssu72 leads to increased synthesis of promoter-associated divergent ncRNAs, referred to as Ssu72-restricted transcripts (SRTs). Similarly, inactivation of individual gene loops by gene mutation enhances SRT synthesis. We demonstrate that gene-loop conformation enforces transcriptional directionality on otherwise bidirectional promoters.
Sue Mei Tan-Wong; Judith B Zaugg; Jurgi Camblong; Zhenyu Xu; David W Zhang; Hannah E Mischo; Aseem Z Ansari; Nicholas M Luscombe; Lars M Steinmetz; Nick J Proudfoot
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-09-27
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  338     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-02     Completed Date:  2012-11-19     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  671-5     Citation Subset:  IM    
Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.
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MeSH Terms
Exosome Multienzyme Ribonuclease Complex / metabolism
Genes, Fungal*
Genome, Fungal
Nucleic Acid Conformation
Phosphoprotein Phosphatases / metabolism
Promoter Regions, Genetic
RNA Polymerase II / metabolism
RNA Stability
RNA, Fungal / genetics,  metabolism
RNA, Messenger / genetics*,  metabolism
RNA, Untranslated / genetics*,  metabolism
Saccharomyces cerevisiae / genetics*,  metabolism
Saccharomyces cerevisiae Proteins / metabolism
Transcription, Genetic*
mRNA Cleavage and Polyadenylation Factors / metabolism
Grant Support
091805//Wellcome Trust; //Wellcome Trust
Reg. No./Substance:
0/RNA, Fungal; 0/RNA, Messenger; 0/RNA, Untranslated; 0/SSU72 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/mRNA Cleavage and Polyadenylation Factors; EC 2.7.7.-/RNA Polymerase II; EC 3.1.-/Exosome Multienzyme Ribonuclease Complex; EC 3.1.13.-/RRP6 protein, S cerevisiae; EC Phosphatases
Comment In:
Science. 2012 Nov 2;338(6107):624-5   [PMID:  23118179 ]
Dev Cell. 2012 Nov 13;23(5):919-21   [PMID:  23153489 ]

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