Document Detail

Gene expression profiling of blood for the prediction of ischemic stroke.
MedLine Citation:
PMID:  20798371     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND PURPOSE: A blood-based biomarker of acute ischemic stroke would be of significant value in clinical practice. This study aimed to (1) replicate in a larger cohort our previous study using gene expression profiling to predict ischemic stroke; and (2) refine prediction of ischemic stroke by including control groups relevant to ischemic stroke.
METHODS: Patients with ischemic stroke (n=70, 199 samples) were compared with control subjects who were healthy (n=38), had vascular risk factors (n=52), and who had myocardial infarction (n=17). Whole blood was drawn ≤3 hours, 5 hours, and 24 hours after stroke onset and from control subjects. RNA was processed on whole genome microarrays. Genes differentially expressed in ischemic stroke were identified and analyzed for predictive ability to discriminate stroke from control subjects.
RESULTS: The 29 probe sets previously reported predicted a new set of ischemic strokes with 93.5% sensitivity and 89.5% specificity. Sixty- and 46-probe sets differentiated control groups from 3-hour and 24-hour ischemic stroke samples, respectively. A 97-probe set correctly classified 86% of ischemic strokes (3 hour+24 hour), 84% of healthy subjects, 96% of vascular risk factor subjects, and 75% with myocardial infarction.
CONCLUSIONS: This study replicated our previously reported gene expression profile in a larger cohort and identified additional genes that discriminate ischemic stroke from relevant control groups. This multigene approach shows potential for a point-of-care test in acute ischemic stroke.
Boryana Stamova; Huichun Xu; Glen Jickling; Cheryl Bushnell; Yingfang Tian; Bradley P Ander; Xinhua Zhan; Dazhi Liu; Renee Turner; Peter Adamczyk; Jane C Khoury; Arthur Pancioli; Edward Jauch; Joseph P Broderick; Frank R Sharp
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-08-26
Journal Detail:
Title:  Stroke; a journal of cerebral circulation     Volume:  41     ISSN:  1524-4628     ISO Abbreviation:  Stroke     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-29     Completed Date:  2010-10-19     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  0235266     Medline TA:  Stroke     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2171-7     Citation Subset:  IM    
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MeSH Terms
Aged, 80 and over
Biological Markers / blood
Brain Ischemia / blood,  genetics*
Gene Expression Profiling*
Middle Aged
Oligonucleotide Array Sequence Analysis
Predictive Value of Tests
Risk Factors
Stroke / blood,  genetics*
Grant Support
NS056302/NS/NINDS NIH HHS; P021040N635110//PHS HHS; P50 NS044283/NS/NINDS NIH HHS; R01 NS056302/NS/NINDS NIH HHS; R01 NS075035/NS/NINDS NIH HHS; UL1 RR024146/RR/NCRR NIH HHS; //Canadian Institutes of Health Research
Reg. No./Substance:
0/Biological Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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