| The gene expression of the main lipogenic enzymes is downregulated in visceral adipose tissue of obese subjects. | |
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MedLine Citation:
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PMID: 19543203 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Contradictory findings regarding the gene expression of the main lipogenic enzymes in human adipose tissue depots have been reported. In this cross-sectional study, we aimed to evaluate the mRNA expression of fatty acid synthase (FAS) and acetyl-CoA carboxilase (ACC) in omental and subcutaneous (SC) fat depots from subjects who varied widely in terms of body fat mass. FAS and ACC gene expression were evaluated by real time-PCR in 188 samples of visceral adipose tissue which were obtained during elective surgical procedures in 119 women and 69 men. Decreased sex-adjusted FAS (-59%) and ACC (-49%) mRNA were found in visceral adipose tissue from obese subjects, with and without diabetes mellitus type 2 (DM-2), compared with lean subjects (both P < 0.0001). FAS mRNA was also decreased (-40%) in fat depots from overweight subjects (P < 0.05). Indeed, FAS mRNA was significantly and positively associated with ACC gene expression (r = 0.316, P < 0.0001) and negatively with BMI (r = -0.274), waist circumference (r = -0.437), systolic blood pressure (r = -0.310), serum glucose (r = -0.277), and fasting triglycerides (r = -0.226), among others (all P < 0.0001). Similar associations were observed for ACC gene expression levels. In a representative subgroup of nonobese (n = 4) and obese women (n = 6), relative FAS gene expression levels significantly correlated (r = 0.657, P = 0.034; n = 10) with FAS protein values. FAS protein levels were also inversely correlated with blood glucose (r = -0.640, P = 0.046) and fasting triglycerides (r = -0.832, P = 0.010). In conclusion, the gene expression of the main lipogenic enzymes is downregulated in visceral adipose tissue from obese subjects. |
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Authors:
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Francisco J Ortega; Dolores Mayas; Jos? M Moreno-Navarrete; Victoria Catal?n; Javier G?mez-Ambrosi; Eduardo Esteve; Jose I Rodriguez-Hermosa; Bartomeu Ruiz; Wifredo Ricart; Belen Peral; Gema Fruhbeck; Francisco J Tinahones; Jos? M Fern?ndez-Real |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-18 |
Journal Detail:
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Title: Obesity (Silver Spring, Md.) Volume: 18 ISSN: 1930-7381 ISO Abbreviation: Obesity (Silver Spring) Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-25 Completed Date: 2010-03-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101264860 Medline TA: Obesity (Silver Spring) Country: United States |
Other Details:
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Languages: eng Pagination: 13-20 Citation Subset: IM |
Affiliation:
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Service of Diabetes, Endocrinology and Nutrition, Institut d'Investigaci? Biom?dica de Girona (IdIBGi), Girona, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetyl-CoA Carboxylase
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genetics*,
metabolism Analysis of Variance Blood Glucose / genetics Blood Pressure / genetics Blotting, Western Body Mass Index Cross-Sectional Studies Diabetes Mellitus, Type 2 / enzymology*, genetics Down-Regulation Fatty Acid Synthetase Complex / genetics*, metabolism Female Gene Expression Gene Expression Regulation, Enzymologic Humans Intra-Abdominal Fat / enzymology* Lipids / blood Male Obesity / enzymology*, genetics Omentum / enzymology RNA, Messenger / genetics, metabolism Regression Analysis Reverse Transcriptase Polymerase Chain Reaction Sex Factors Subcutaneous Fat / enzymology Waist Circumference / genetics |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Lipids; 0/RNA, Messenger; EC 6.-/Fatty Acid Synthetase Complex; EC 6.4.1.2/Acetyl-CoA Carboxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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