Document Detail

Gene Expression Analysis of Membrane Transport Proteins in Normal and Lipopolysaccharide-inflamed Rat Dental Pulp.
MedLine Citation:
PMID:  22515894     Owner:  NLM     Status:  In-Data-Review    
INTRODUCTION: Membrane transport proteins (transporters) play a crucial role in the transmembrane uptake and/or efflux of various compounds such as inorganic ions, endogenous bioactive substances such as prostaglandins (PGs), and drugs such as nonsteroidal anti-inflammatory drugs. This study aimed to analyze mRNA expression of selected transporters related to drug disposition and PG transport in normal and lipopolysaccharide (LPS)-inflamed rat incisor pulp.
METHODS: Pulp tissues were subjected to reverse transcription-polymerase chain reaction (PCR) detection for transporter isoforms belonging to organic anion transporting polypeptide (Oatp), organic anion transporter (Oat), organic cation transporter (Oct), multidrug resistance-associated protein (Mrp), and multidrug resistance protein (Mdr) families. The levels of mRNA expression for PG transporters (Oatp1a5, Oatp1b2, Oatp2a1, Oatp2b1, and Oatp3a1) were compared in normal and LPS-inflamed pulps by using real-time PCR.
RESULTS: The pulp tissue expressed mRNAs for various transporters belonging to the Oatp, Oat, Oct, Mrp, and Mdr families. LPS inflammation caused significant up-regulation of Oatp2a1 (P < .01) and significant down-regulation of Oatp1a5, Oatp2b1 (P < .01), and Oatp3a1 (P < .05).
CONCLUSIONS: Rat incisor dental pulp expressed mRNAs for various transporter isoforms. The levels of mRNA expression for PG transporters were significantly up-regulated or down-regulated in LPS-inflamed dental pulp.
Naoto Ohkura; Yoshimi Shigetani; Nagako Yoshiba; Kunihiko Yoshiba; Takashi Okiji
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Publication Detail:
Type:  Journal Article     Date:  2012-03-27
Journal Detail:
Title:  Journal of endodontics     Volume:  38     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  648-52     Citation Subset:  D    
Copyright Information:
Copyright © 2012 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.
Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
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