Document Detail


Gender-related differences in muscle injury, oxidative stress, and apoptosis.
MedLine Citation:
PMID:  18799987     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Due to its alleged antioxidant properties, 17beta-estradiol (E2) may protect against muscle injury, oxidative stress, and apoptosis. PURPOSE: This study sought to determine whether such mechanisms existed between genders for muscle injury, oxidative stress, and apoptosis after eccentric exercise. METHODS: Eight men and eight women (no oral contraceptive use; midluteal phase of menstrual cycle) performed 7 x 10 eccentric repetitions of the knee extensors at 150% 1RM. Strength, soreness, and blood samples were taken before exercise and 6, 24, 48, and 72 h after exercise while muscle samples were collected before and 6 and 24 h after exercise. Blood samples were assayed for free E2, lactate dehydrogenase (LDH), superoxide dismutase (SOD), and 8-isoprostane (8-iso). Muscle samples were assayed for mitochondrial apoptosis (e.g., bax, bcl-2, cytochrome c, and cell death), total DNA content, and myofibrillar protein content. RESULTS: Men reported greater soreness levels at 24, 48, and 72 h after exercise, whereas strength changes were similar among genders. At baseline and independent of exercise, females had higher E2 (P < 0.001) and SOD in conjunction with lower 8-iso levels when compared with men. Bax increased in both genders, whereas bcl-2 increased only in women with no cytochrome c changes for either gender after exercise. The bax/bcl-2 ratio in women significantly decreased after 6 h (P = 0.03) and returned to baseline levels after 24 h. Men exhibited greater cell death at all time points (P < 0.05), whereas myofibrillar protein content and total DNA content decreased in both genders at 24 h after exercise. No changes in LDH were found (P > 0.05). CONCLUSIONS: Although more research is needed, differences between gender may provide greater endogenous protection against oxidative stress and apoptosis.
Authors:
Chad Kerksick; Lem Taylor; Alison Harvey; Darryn Willoughby
Related Documents :
12788477 - Effect of vitamin e and eccentric exercise on selected biomarkers of oxidative stress i...
16825267 - Decrease in eccentric hamstring strength in runners in the tirol speed marathon.
17569697 - Creatine kinase monitoring in sport medicine.
10919967 - Does dietary creatine supplementation play a role in skeletal muscle metabolism and per...
16343807 - Duration ratio discrimination in pigeons: a criterion-setting analysis.
8792347 - Physical exercise as an oncology nursing intervention to enhance quality of life.
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  40     ISSN:  1530-0315     ISO Abbreviation:  Med Sci Sports Exerc     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-18     Completed Date:  2008-12-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1772-80     Citation Subset:  IM; S    
Affiliation:
Applied Biochemistry and Molecular Physiology Laboratory, Health and Exercise Science Department, University of Oklahoma, Norman, OK, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Apoptosis*
Biopsy, Needle
Body Composition
DNA / metabolism
Dinoprost / analogs & derivatives,  blood
Enzyme-Linked Immunosorbent Assay
Estradiol / blood,  physiology
Exercise
Female
Humans
L-Lactate Dehydrogenase / blood
Male
Muscle Proteins / metabolism
Muscle, Skeletal / injuries*,  metabolism,  pathology
Oxidative Stress*
Sex Factors*
Superoxide Dismutase / blood
Chemical
Reg. No./Substance:
0/Muscle Proteins; 27415-26-5/8-epi-prostaglandin F2alpha; 50-28-2/Estradiol; 551-11-1/Dinoprost; 9007-49-2/DNA; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.15.1.1/Superoxide Dismutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Relationship between physical activity level, telomere length, and telomerase activity.
Next Document:  Weight regain is related to decreases in physical activity during weight loss.