Document Detail

Gender differences in small intestinal endothelial function: inhibitory role of androgens.
MedLine Citation:
PMID:  14563675     Owner:  NLM     Status:  MEDLINE    
Although gender differences exist in cardiovascular endothelial function, it remains unclear whether such differences are also seen in small intestinal endothelial function. To determine this, untreated male, age-matched proestrus female, castrated male, and 17beta-estradiol (E2)-treated noncastrated male rats were studied. Dose response curves to ACh and nitroglycerin (NTG) were determined by measuring changes in perfusion pressure by using an isolated small intestinal perfusion model. Endothelium-derived nitric oxide (NO) production/release was indirectly determined by the ability of intact endothelium to suppress serotonin (10(-5) M)-induced perfusion pressure changes. Intestinal tissue levels of NO were also measured. Moreover, plasma levels of androgen and E2 were determined and correlated with ACh (10(-8) M)-induced perfusion pressure reductions. ACh-induced intestinal perfusion pressure reductions in proestrus females, castrated males, and E2-treated noncastrated males were significantly higher than in untreated males. NTG-induced perfusion pressure reductions were not significantly different among groups. Perfusion pressures after administration of serotonin (10(-5) M) and intestinal tissue levels of NO in proestrus females, castrated males, and E2-treated noncastrated males were also significantly higher than in untreated males. Plasma androgen levels in proestrus females, castrated males, and in E2-treated noncastrated males were significantly lower compared with untreated males. There was a positive correlation between plasma androgen and ACh-reduced perfusion pressure; however, E2 levels did not show a similar relationship. Thus androgens appear to play an inhibitory role in small intestinal endothelial function. These properties in male vessels can be modulated by decreasing the level of circulating androgens or by E2 treatment.
Zheng F Ba; Yukihiro Yokoyama; Balazs Toth; Loring W Rue; Kirby I Bland; Irshad H Chaudry
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2003-10-16
Journal Detail:
Title:  American journal of physiology. Gastrointestinal and liver physiology     Volume:  286     ISSN:  0193-1857     ISO Abbreviation:  Am. J. Physiol. Gastrointest. Liver Physiol.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-09     Completed Date:  2004-03-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901227     Medline TA:  Am J Physiol Gastrointest Liver Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  G452-7     Citation Subset:  IM    
Center for Surgical Research and Department of Surgery, University of Alabama at Birmingham, 35294-0019, USA.
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MeSH Terms
Acetylcholine / metabolism
Androgens / blood,  pharmacology*
Blood Pressure / drug effects
Dihydrotestosterone / blood
Endothelium / drug effects,  physiology
Estradiol / blood,  pharmacology
Estrogens / blood
Intestine, Small / drug effects,  physiology*
Nitric Oxide / biosynthesis
Nitroglycerin / pharmacology
Proestrus / physiology
Rats, Sprague-Dawley
Sex Characteristics
Testosterone / blood
Vasodilator Agents / pharmacology
Grant Support
Reg. No./Substance:
0/Androgens; 0/Estrogens; 0/Vasodilator Agents; 10102-43-9/Nitric Oxide; 50-28-2/Estradiol; 51-84-3/Acetylcholine; 521-18-6/Dihydrotestosterone; 55-63-0/Nitroglycerin; 58-22-0/Testosterone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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