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Gender-dependent metabolic remodeling during heart preservation in cardioplegic Celsior and Histidine-buffer solution.
MedLine Citation:
PMID:  21983744     Owner:  NLM     Status:  Publisher    
ABSTRACT: Understanding heart metabolism during preservation is crucial to develop new effective cardioplegic solutions. We aim to investigate metabolic alterations during heart preservation in the clinically used Celsior (Cs) and Histidine Buffer solution (HBS). We also focused in gender-specific metabolic adaptations during ischemia.We followed energy metabolism in hearts from males and females preserved during 6 hours in Cs and HBS. Hearts were subjected to cold ischemia (4°C) in Cs or HBS and aliquots of the cardioplegic solution were collected throughout preservation for nuclear magnetic resonance analysis.HBS-preserved hearts from males consumed glucose mostly between 240-360 min while HBS-preserved hearts from females consumed glucose throughout the 6 hours of ischemia. Lactate production rates followed approximately the glucose consumption rates in HBS-preserved hearts. The lactate/alanine ratio, an indicator of the redox state, was increased in HBS-preserved hearts when compared with Cs-preserved hearts. Hearts from males presented a higher redox state than hearts from females preserved in Cs after 300 min. Both, Cs and HBS, were capable of preventing acidification in hearts from females but not in hearts from males which decreased the extracellular pH. HBS-preserved hearts from males and females produced 0.1±0.01 and 0.15±0.03 μmol/min/gdw of lactate, respectively. Those rates were significantly higher than in Cs-preserved hearts. Thus, Cs was more effective in preventing lactate production.We conclude that glycolysis and lactate production are stimulated in HBS-preserved hearts. HBS shows better overall results particularly in hearts from females which presented less extracellular acidification and were more effective in recycling the metabolic sub-products.
Marco G Alves; Pedro F Oliveira; Fátima O Martins; Paulo J Oliveira; Rui A Carvalho
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-10-6
Journal Detail:
Title:  Journal of cardiovascular pharmacology     Volume:  -     ISSN:  1533-4023     ISO Abbreviation:  -     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7902492     Medline TA:  J Cardiovasc Pharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
aCNC - Center for Neuroscience and Cell Biology, University of Coimbra, 3004-517 Coimbra - Portugal bDepartment of Life Sciences, Faculty of Science and Technology, University of Coimbra, 3004-517 Coimbra - Portugal cCICS-UBI, Health Sciences Research Center, University of Beira Interior, 6200-506 Covilhã - Portugal.
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